Comparison of Established PCR Assays for Accurate Identification of Campylobacter jejuni and Campylobacter coli

被引:7
|
作者
Kabir, S. M. Lutful [1 ,2 ]
Chowdhury, Nityananda [1 ,4 ]
Asakura, Masahiro [1 ]
Shiramaru, Sachi [1 ]
Kikuchi, Ken [3 ,5 ]
Hinenoya, Atsushi [1 ]
Neogi, Sucharit Basu [1 ]
Yamasaki, Shinji [1 ]
机构
[1] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, izu, 1-58 Rinku Ourai Kita, Izumisano, Osaka 5988531, Japan
[2] Bangladesh Agr Univ, Dept Microbiol & Hyg, Mymensingh, Bangladesh
[3] Juntendo Univ, Fac Med, Dept Infect Control Sci, Tokyo 1138421, Japan
[4] Med Univ South Carolina, Charleston, SC 29425 USA
[5] Tokyo Womens Med Univ, Grad Sch Med Sci, Tokyo, Japan
基金
日本学术振兴会;
关键词
SPECIES-LEVEL; GENE; STRAINS; DIFFERENTIATION; LARI; VIRULENCE; FETUS;
D O I
10.7883/yoken.JJID.2018.340
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Proper surveillance of Campylobacter jejuni and Campylobacter coli, major pathogens associated with human gastroenteritis, is necessary to tackle the increasing disease burden. To detect these pathogenic species, a variety of PCR assays have been developed. This study examined the sensitivity and specificity of 12 PCR assays targeting 23S rRNA, ceuE, lpxA, hipO, mapA, ask, and cdt genes of C. jejuni and C. coli. The sensitivities of PCR assays were 85.2-100%, and 97-100%, and the specificities were 90.5-100%, and 94.3-100% for the tested C. jejuni (n = 61) and C. coli (n = 33) strains, respectively. Two PCR assays, targeting cdtC and hipO genes, were found to be 100% sensitive and/or specific for all C. jejuni strains, while 3 assays, targeting cdtB, cdtA, and ask genes, were 100% sensitive and/or specific for C. coli strains. However, PCR assays for hipO and ask genes are problematic to conduct simultaneously due to the differences in PCR conditions. Overall, multiplex PCR assays targeting cdtC and cdtB genes, encoding 2 subunits of the same toxin, were concluded to be the most reliable. The results of this study would aid in proper surveillance of C. jejuni and C. coli and adopting intervention strategies in the near future.
引用
收藏
页码:81 / 87
页数:7
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