PRODUCTION OF ANTIBODIES AGAINST HER2 AND HER3 RECEPTORS IN BREAST CANCER TREATMENT AND THE SUPERIOR EFFECT OF THE SYNCHRONOUS BLOCKADE OF THESE RECEPTORS

Background: The human epidermal growth factor receptor family (ErbB receptor family) plays an important role in the development of cancer. Antibody phage display technique is one of the powerful techniques that allows highly specific selection for antibody selection. Materials and Methods: In this study, by using antibody phage display technique, which is one of the powerful techniques for making antibodies in vitro, recombinant antibodies against ErbB2 and ErbB3 receptors were produced for inhibition of these receptors and compared the simultaneous blockage of these receptors with a synchronous block of HER2 and HER3. Results: We established a panel of stable cell lines expressing high levels of HER2 and HER3 and then we have produced antibodies with phage display technique against ErbB2 and ErbB3 receptors and compared the simultaneous blockage of these receptors with a synchronous block of HER2 and HER3. Conclusions: The specificity of antibodies obtained in this method was greatly improved and we've shown that synchronous block of HER2 and HER3 receptors has a superior effect compared with the monospecific block.