METHOTREXATE POTENTIATES ANTI-HERPES SIMPLEX VIRUS TYPE-1 ACTIVITY OF E-5-(2-BROMOVINYL)-2'-DEOXYURIDINE

被引：0
|
作者：
PANCHEVA, SN
机构：
来源：
ACTA VIROLOGICA | 1995年 / 39卷 / 02期
关键词：
METHOTREXATE; BROMOVINYLDEOXYURIDINE; ANTIVIRALS; SYNERGISM; HERPES SIMPLEX VIRUS TYPE 1;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The pharmacologic of induction of low level of deoxyribanucleoside triphosphates (dNTP) is a novel approach to combined inhibition of virus replication. In accordance with this concept the alteration of antiherpes activity of E-5-(2-bromovinyl-2'-deoxyuridine (BVDU) in combination with methotrexate (MTX), an inhibitor which lowers the intracellular pool of thymidine triphosphate (dTTP) was studied. MTX applied alone at non-toxic concentrations had a moderate effect on herpes simplex virus type 1 (HSV-1) replication in human embryonic skin-muscle fibroblasts. The combination of BVDU and MTX acts synergistically, as measured by virus yield assay in the above mentioned system. The potentiating effect of MTX on the anti-HSV-1 activity of BVDU was reversed by thymidine (dThd).
引用
收藏
页码：117 / 119
页数：3
相关论文
共 50 条
  • [41] INHIBITORY EFFECT OF E-5-(2-BROMOVINYL)-1-BETA-D-ARABINOFURANOSYLURACIL ON HERPES-SIMPLEX VIRUS-REPLICATION AND DNA-SYNTHESIS
    DESCAMPS, J
    SEHGAL, RK
    DECLERCQ, E
    ALLAUDEEN, HS
    JOURNAL OF VIROLOGY, 1982, 43 (01) : 332 - 336
  • [42] Anti-herpes simplex virus type-1 activity of Eleusine indica methanol extract
    Iberahim, Rashidah
    Bahtiar, Adibah Ahamad
    Ibrahim, Nazlina
    MALAYSIAN JOURNAL OF MICROBIOLOGY, 2016, 12 (06) : 471 - 474
  • [43] IDENTIFICATION AND TYPING OF HERPES-SIMPLEX VIRUS TYPE-1 AND TYPE-2 BY MONOCLONAL-ANTIBODIES, SENSITIVITY TO THE DRUG (E)-5-(2-BROMOVINYL)-2'-DEOXYURIDINE, AND RESTRICTION ENDONUCLEASE ANALYSIS OF VIRAL-DNA
    ZIMMERMAN, DH
    MUNDON, FK
    CROSON, SE
    HENCHAL, LS
    DOCHERTY, JJ
    ONEILL, SP
    JOURNAL OF MEDICAL VIROLOGY, 1985, 15 (03) : 215 - 222
  • [44] EFFECTS OF VARIOUS NUCLEOSIDES ON ANTIVIRAL ACTIVITY AND METABOLISM OF 1-BETA-D-ARABINOFURANOSYL-E-5-(2-BROMOVINYL)URACIL AGAINST HERPES-SIMPLEX VIRUS TYPE-1 AND TYPE-2
    SUZUTANI, T
    MACHIDA, H
    SAKUMA, T
    AZUMA, M
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1988, 32 (10) : 1547 - 1551
  • [45] EFFECT OF 1-BETA-D-ARABINOFURANOSYL-E-5-(2-BROMOVINYL)URACIL AGAINST HERPES-SIMPLEX VIRUS TYPE-1 INFECTION IN IMMUNOSUPPRESSED MICE
    IJICHI, K
    ASHIDA, N
    MACHIDA, H
    ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1990, 34 (12) : 2431 - 2433
  • [46] Seaweeds with anti-herpes simplex virus type 1 activity
    Barros, Caroline de Souza
    Teixeira, Valeria Laneuville
    Paixao, Izabel Christina N. P.
    JOURNAL OF APPLIED PHYCOLOGY, 2015, 27 (04) : 1623 - 1637
  • [47] Seaweeds with anti-herpes simplex virus type 1 activity
    Caroline de Souza Barros
    Valéria Laneuville Teixeira
    Izabel Christina N. P. Paixão
    Journal of Applied Phycology, 2015, 27 : 1623 - 1637
  • [48] Different mechanisms of inhibition of DNA synthesis by (E)-5-(2-bromovinyl)-2'-deoxyuridine in cells transfected with gene for thymidine kinase of herpes simplex virus type 1 and in cells infected with the virus
    Hirota, Y
    Arao, Y
    Matsumoto, A
    Nii, S
    Wataya, Y
    NUCLEOSIDES & NUCLEOTIDES, 1995, 14 (9-10): : 2079 - 2087
  • [49] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ANALYSIS OF (E)-5-(2-BROMOVINYL)-2'-DEOXYURIDINE AND ITS METABOLITES IN SERUM, URINE AND HERPES-SIMPLEX VIRUS TYPE-1 INFECTED-CELLS
    AYISI, NK
    WALL, RA
    DECLERCQ, E
    SACKS, SL
    JOURNAL OF CHROMATOGRAPHY, 1986, 375 (02): : 423 - 430
  • [50] DIFFERENTIAL METABOLISM OF (E)-5-(2-BROMOVINYL)-2'-DEOXYURIDINE IN WILD-TYPE AND DRUG-RESISTANT HERPES-SIMPLEX VIRUS-INFECTED CELLS
    AYISI, N
    WALL, R
    DECLERCQ, E
    SACKS, S
    ANTIVIRAL RESEARCH, 1985, : 45 - 49
12345