METHOTREXATE POTENTIATES ANTI-HERPES SIMPLEX VIRUS TYPE-1 ACTIVITY OF E-5-(2-BROMOVINYL)-2'-DEOXYURIDINE

被引：0
|
作者：
PANCHEVA, SN
机构：
来源：
ACTA VIROLOGICA | 1995年 / 39卷 / 02期
关键词：
METHOTREXATE; BROMOVINYLDEOXYURIDINE; ANTIVIRALS; SYNERGISM; HERPES SIMPLEX VIRUS TYPE 1;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The pharmacologic of induction of low level of deoxyribanucleoside triphosphates (dNTP) is a novel approach to combined inhibition of virus replication. In accordance with this concept the alteration of antiherpes activity of E-5-(2-bromovinyl-2'-deoxyuridine (BVDU) in combination with methotrexate (MTX), an inhibitor which lowers the intracellular pool of thymidine triphosphate (dTTP) was studied. MTX applied alone at non-toxic concentrations had a moderate effect on herpes simplex virus type 1 (HSV-1) replication in human embryonic skin-muscle fibroblasts. The combination of BVDU and MTX acts synergistically, as measured by virus yield assay in the above mentioned system. The potentiating effect of MTX on the anti-HSV-1 activity of BVDU was reversed by thymidine (dThd).
引用
收藏
页码：117 / 119
页数：3
相关论文
共 50 条
  • [31] MODIFIED POLYNUCLEOTIDES .6. PROPERTIES OF A SYNTHETIC DNA CONTAINING THE ANTI-HERPES AGENT (E)-5-(2-BROMOVINYL)-2'-DEOXYURIDINE
    SAGI, J
    CZUPPON, A
    KAJTAR, M
    SZABOLCS, A
    SZEMZO, A
    OTVOS, L
    NUCLEIC ACIDS RESEARCH, 1982, 10 (19) : 6051 - 6066
  • [32] TREATMENT OF EXPERIMENTAL HERPES-SIMPLEX VIRUS TYPE-1 ENCEPHALITIS IN MICE WITH (E)-5-(2-BROMOVINYL)- AND 5-VINYL-1-BETA-D-ARABINO-FURANOSYLURACIL - COMPARISON WITH BROMOVINYL-DEOXYURIDINE AND ACYCLOVIR
    REEFSCHLAGER, J
    WUTZLER, P
    THIEL, KD
    HERRMANN, G
    ANTIVIRAL RESEARCH, 1986, 6 (02) : 83 - 93
  • [33] BIOANALYSIS OF (E)-5-(2-BROMOVINYL)-2'-DEOXYURIDINE
    REEUWIJK, HJEM
    LINGEMAN, H
    TJADEN, UR
    DEBRUIJN, EA
    KEIZER, HJ
    VANDERGREEF, J
    JOURNAL OF CHROMATOGRAPHY-BIOMEDICAL APPLICATIONS, 1988, 428 (01): : 93 - 102
  • [34] (E)5-(2-bromovinyl)-2′ deoxyuridine (BVDU)
    De Clercq, E
    MEDICINAL RESEARCH REVIEWS, 2005, 25 (01) : 1 - 20
  • [35] INDUCTION OF NUCLEAR DEOXYRIBOPYRIMIDINE TRIPHOSPHATASE AND SENSITIVITY OF CLINICAL ISOLATES OF HERPES-SIMPLEX VIRUS TO (E)-5-(2-BROMOVINYL)-2'-DEOXYURIDINE
    WOHLRAB, F
    MAYO, DR
    HSIUNG, GD
    FRANCKE, B
    ANTIVIRAL RESEARCH, 1983, 3 (01) : 1 - 5
  • [36] ORAL (E)-5-(2-BROMOVINYL)-2'-DEOXYURIDINE IN SEVERE HERPES-ZOSTER
    DECLERCO, E
    DEGREEF, H
    WILDIERS, J
    DEJONGE, G
    DROCHMANS, A
    DESCAMPS, J
    DESOMER, P
    BRITISH MEDICAL JOURNAL, 1980, 281 (6249): : 1178 - 1178
  • [37] (E)-5-(2-BROMOVINYL)URIDINE REQUIRES PHOSPHORYLATION BY THE HERPES-SIMPLEX VIRUS (TYPE-1)-INDUCED THYMIDINE KINASE TO EXPRESS ITS ANTIVIRAL ACTIVITY
    BERNAERTS, R
    DESGRANGES, C
    DECLERCQ, E
    BIOCHEMICAL PHARMACOLOGY, 1989, 38 (12) : 1955 - 1961
  • [38] THERAPEUTIC EFFECT OF (E)-5-(2-BROMOVINYL)-2'-DEOXYURIDINE, CAFFEIC ACID OXIDATION-PRODUCT, AND TRISODIUMPHOSPHONOFORMATE ON CUTANEOUS HERPES-SIMPLEX VIRUS TYPE-1 INFECTION IN GUINEA-PIGS
    HELBIG, B
    SAUERBREI, A
    KLOCKING, R
    WUTZLER, P
    WICHT, N
    WIEDEMANN, U
    HERRMANN, G
    JOURNAL OF MEDICAL VIROLOGY, 1987, 23 (03) : 303 - 309
  • [39] SYNTHESIS, MOLECULAR CONFORMATION AND ACTIVITY AGAINST HERPES SIMPLEX VIRUS OF (E)-5-(2-BROMOVINYL)-2′-DEOXYCYTIDINE ANALOGS
    Zoghaib, Wajdi Michel
    Mannala, Shajan
    Gupta, V. Sagar
    Tourigny, Guy
    NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 2012, 31 (04): : 364 - 376
  • [40] EFFECTS OF (E)-5-(2-BROMOVINYL)-2'-DEOXYURIDINE ON THE PROLIFERATION OF HERPES-SIMPLEX VIRUS TYPE-1-TRANSFORMED AND THYMIDINE KINASE-DEFICIENT MOUSE CELLS
    BUBLEY, G
    CRUMPACKER, C
    DECLERCQ, E
    SCHNIPPER, L
    VIROLOGY, 1983, 129 (02) : 490 - 492
12345