DIRECT SEQUENCING OF PCR PRODUCTS USING THE MAXAM-GILBERT METHOD

被引：3
|
作者：
STAMM, S [1 ]
LONGO, FM [1 ]
机构：
[1] UNIV CALIF SAN FRANCISCO,DEPT NEUROL,SAN FRANCISCO,CA 94143
来源：
GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1990年 / 7卷 / 05期
关键词：
D O I：
10.1016/0735-0651(90)90021-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Direct sequencing of polymerase chain reaction (PCR) products by using the Maxam-Gilbert method is described. In this method, one of the primers is end labeled. Thus it is possible to sequence the reaction product directly following purification using this chemical method. © 1990.
引用
收藏
页码：142 / 143
页数：2
相关论文
共 50 条
  • [41] Resolution of DRB1 ambiguous heterozygotes by direct sequencing of PCR products.
    McGinnis, MD
    Brown, CL
    Conrad, MP
    Iovannisci, DM
    KuKuruga, D
    Rosemuller, EH
    Tilanus, MGJ
    Kronick, MN
    HUMAN IMMUNOLOGY, 1996, 49 : 166 - 166
  • [42] Preparation of PCR products for DNA sequencing
    Leonard, JT
    Grace, MB
    Buzard, GS
    Mullen, MJ
    Barbagallo, CB
    BIOTECHNIQUES, 1998, 24 (02) : 314 - 317
  • [43] Comprehensive method for the typing of HLA-A, B, and C alleles by direct sequencing of PCR products obtained from genomic DNA
    Bettinotti, MP
    Mitsuishi, Y
    Bibee, K
    Lau, M
    Terasaki, PI
    JOURNAL OF IMMUNOTHERAPY, 1997, 20 (06): : 425 - 430
  • [44] Direct PCR sequencing with boronated nucleotides
    Porter, KW
    Briley, JD
    Shaw, BR
    NUCLEIC ACIDS RESEARCH, 1997, 25 (08) : 1611 - 1617
  • [45] DIRECT SEQUENCING BY THERMAL ASYMMETRIC PCR
    MAZARS, GR
    MOYRET, C
    JEANTEUR, P
    THEILLET, CG
    NUCLEIC ACIDS RESEARCH, 1991, 19 (17) : 4783 - 4783
  • [46] A RAPID METHOD FOR PURIFYING PCR PRODUCTS FOR DIRECT SEQUENCE-ANALYSIS
    KALNOSKI, MH
    MCCOYHAMAN, MF
    HOLLIS, GF
    BIOTECHNIQUES, 1991, 11 (02) : 246 - &
  • [47] DIRECT SEQUENCING OF LARGE FLAVIVIRUS PCR PRODUCTS FOR ANALYSIS OF GENOME VARIATION AND MOLECULAR EPIDEMIOLOGIC INVESTIGATIONS
    LEWIS, JG
    CHANG, GJ
    LANCIOTTI, RS
    TRENT, DW
    JOURNAL OF VIROLOGICAL METHODS, 1992, 38 (01) : 11 - 23
  • [48] DIRECT SEQUENCING OF DOUBLE-STRANDED PCR PRODUCTS ISOLATED FROM CONVENTIONAL AGAROSE GELS
    CAO, XN
    BROSIUS, FC
    BIOTECHNIQUES, 1993, 15 (03) : 384 - 385
  • [49] Identifying DNA polymorphisms in human TCRA/D variable genes by direct sequencing of PCR products
    Boysen, C
    Carlson, C
    Hood, E
    Hood, L
    Nickerson, DA
    IMMUNOGENETICS, 1996, 44 (02) : 121 - 127
  • [50] A SIMPLE AND RAPID METHOD OF DIRECT SEQUENCING USING DYNABEADS
    THEIN, SL
    HINTON, J
    BRITISH JOURNAL OF HAEMATOLOGY, 1991, 79 (01) : 113 - 115
12345