DIRECT SEQUENCING OF PCR PRODUCTS USING THE MAXAM-GILBERT METHOD

被引：3

作者：

STAMM, S ^{[1
]}

LONGO, FM ^{[1
]}

机构：

[1] UNIV CALIF SAN FRANCISCO,DEPT NEUROL,SAN FRANCISCO,CA 94143

来源：

GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1990年 / 7卷 / 05期

关键词：

D O I：

10.1016/0735-0651(90)90021-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Direct sequencing of polymerase chain reaction (PCR) products by using the Maxam-Gilbert method is described. In this method, one of the primers is end labeled. Thus it is possible to sequence the reaction product directly following purification using this chemical method. © 1990.

引用

页码：142 / 143

页数：2

共 50 条

[21] An efficient method for purification of PCR products for sequencing
Ma, Hao
DiFazio, Stephen
BIOTECHNIQUES, 2008, 44 (07) : 921 - 923
[22] DIRECT SEQUENCING OF SINGLE PRIMER PCR PRODUCTS - A RAPID METHOD TO ACHIEVE SHORT CHROMOSOMAL WALKS
SCREATON, GR
BANGHAM, CRM
BELL, JI
NUCLEIC ACIDS RESEARCH, 1993, 21 (09) : 2263 - 2264
[23] DIRECT SEQUENCING OF PCR PRODUCTS IN AGAROSE-GEL SLICES
KHORANA, S
GAGEL, RF
COTE, GJ
NUCLEIC ACIDS RESEARCH, 1994, 22 (16) : 3425 - 3426
[24] Identifying mutations in Drosophila genes by direct sequencing of PCR products
Schlag, EM
Wassarman, DA
BIOTECHNIQUES, 1999, 27 (02) : 262 - +
[25] A SIMPLE METHOD FOR DIRECT AUTOMATED SEQUENCING OF PCR FRAGMENTS
TRACY, TE
MULCAHY, LS
BIOTECHNIQUES, 1991, 11 (01) : 68 - &
[26] The combination of Gilbert/Maxam chemical sequencing and the dideoxynucleotide chain termination approach facilitates the construction of species specific PCR-primers based on diagnostic RAPD bands
Voigt, K
Wostemeyer, J
MICROBIOLOGICAL RESEARCH, 1995, 150 (04) : 373 - 377
[27] GENERAL-METHOD FOR PCR AMPLIFICATION AND DIRECT SEQUENCING OF MESSENGER-RNA DIFFERENTIAL DISPLAY PRODUCTS
REEVES, SA
RUBIO, MP
LOUIS, DN
BIOTECHNIQUES, 1995, 18 (01) : 18 - 20
[28] NONRADIOACTIVE DIRECT SEQUENCING OF PCR PRODUCTS AMPLIFIED FROM NEUROPATHOLOGICAL SPECIMENS
KOSEL, S
GRAEBER, MB
BRAIN PATHOLOGY, 1993, 3 (04) : 421 - 424
[29] RAPID SEQUENCING OF UNPURIFIED PCR PRODUCTS BY THERMAL ASYMMETRIC PCR CYCLE SEQUENCING USING UNLABELED SEQUENCING PRIMERS
LIU, YG
MITSUKAWA, N
WHITTIER, RF
NUCLEIC ACIDS RESEARCH, 1993, 21 (14) : 3333 - 3334
[30] A practical workflow for forensic species identification using direct sequencing of real-time PCR products
Doi, Masanori
Nakagawa, Toshifumi
Asano, Migiwa
MOLECULAR BIOLOGY REPORTS, 2024, 51 (01)

← 1 2 3 4 5 →