DIRECT SEQUENCING OF PCR PRODUCTS USING THE MAXAM-GILBERT METHOD

被引：3
|
作者：
STAMM, S [1 ]
LONGO, FM [1 ]
机构：
[1] UNIV CALIF SAN FRANCISCO,DEPT NEUROL,SAN FRANCISCO,CA 94143
来源：
GENETIC ANALYSIS-BIOMOLECULAR ENGINEERING | 1990年 / 7卷 / 05期
关键词：
D O I：
10.1016/0735-0651(90)90021-7
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Direct sequencing of polymerase chain reaction (PCR) products by using the Maxam-Gilbert method is described. In this method, one of the primers is end labeled. Thus it is possible to sequence the reaction product directly following purification using this chemical method. © 1990.
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页码：142 / 143
页数：2
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