TRANSFORMATION OF THE INDUSTRIAL STRAIN OF ASPERGILLUS-ORYZAE WITH THE HOMOLOGOUS AMDS GENE AS A DOMINANT SELECTABLE MARKER

被引：34
|
作者：
GOMI, K
KITAMOTO, K
KUMAGAI, C
机构：
[1] National Research Institute of Brewing, Kita-ku, Tokyo, 114, 2-6-30, Takinogawa
来源：
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1992年 / 74卷 / 06期
关键词：
D O I：
10.1016/0922-338X(92)90037-U
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A homologous transformation system has been developed for Aspergillus oryzae industrial strains using the A. oryzae acetamidase-encoding gene (amdS) as a dominant selectable marker. Although the transformation efficiency is relatively low (0.4-1 transformants per mug DNA), the transformants contained significantly high copy numbers of the plasmid integrated into the genome. In addition, the promoter of the amdS gene was replaced by the strong expression promoter of the alpha-amylase-encoding gene (amyB) from A. oryzae, thereby enhancing the selection of the transformants.
引用
收藏
页码：389 / 391
页数：3
相关论文
共 50 条
  • [41] TRANSFORMATION OF CULTURED DROSOPHILA-MELANOGASTER CELLS WITH A DOMINANT SELECTABLE MARKER
    RIO, DC
    RUBIN, GM
    MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (08) : 1833 - 1838
  • [42] THE OLIC3 GENE OF ASPERGILLUS-NIGER - ISOLATION, SEQUENCE AND USE AS A SELECTABLE MARKER FOR TRANSFORMATION
    WARD, M
    WILSON, LJ
    CARMONA, CL
    TURNER, G
    CURRENT GENETICS, 1988, 14 (01) : 37 - 42
  • [43] Use of mutated PDR3 gene as a dominant selectable marker in transformation of prototrophic yeast strains
    Lacková, D
    Subík, J
    FOLIA MICROBIOLOGICA, 1999, 44 (02) : 171 - 176
  • [44] PHLEOMYCIN RESISTANCE AS A DOMINANT SELECTABLE MARKER FOR PLANT-CELL TRANSFORMATION
    PEREZ, P
    TIRABY, G
    KALLERHOFF, J
    PERRET, J
    PLANT MOLECULAR BIOLOGY, 1989, 13 (04) : 365 - 373
  • [45] HIGH-LEVEL EXPRESSION OF THE SYNTHETIC HUMAN LYSOZYME GENE IN ASPERGILLUS-ORYZAE
    TSUCHIYA, K
    TADA, S
    GOMI, K
    KITAMOTO, K
    KUMAGAI, C
    JIGAMI, Y
    TAMURA, G
    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1992, 38 (01) : 109 - 114
  • [46] EXPRESSION OF ASPERGILLUS-ORYZAE ALPHA-AMYLASE GENE IN SACCHAROMYCES-CEREVISIAE
    RANDEZGIL, F
    SANZ, P
    FEMS MICROBIOLOGY LETTERS, 1993, 112 (01) : 119 - 123
  • [47] The Aspergillus nidulans amdS gene as a marker for the identification of multicopy T-DNA integration events in Agrobacterium-mediated transformation of Aspergillus awamori
    Michielse, CB
    Ram, AFJ
    van den Hondel, CAMJJ
    CURRENT GENETICS, 2004, 45 (06) : 399 - 403
  • [48] VERMILION AS A SMALL SELECTABLE MARKER GENE FOR DROSOPHILA TRANSFORMATION
    FRIDELL, YWC
    SEARLES, LL
    NUCLEIC ACIDS RESEARCH, 1991, 19 (18) : 5082 - 5082
  • [49] GENOME STRUCTURE AND NUCLEOTIDE-SEQUENCE OF A LIPOLYTIC ENZYME GENE OF ASPERGILLUS-ORYZAE
    OHNISHI, K
    TOIDA, J
    NAKAZAWA, H
    SEKIGUCHI, J
    FEMS MICROBIOLOGY LETTERS, 1995, 126 (02) : 145 - 150
  • [50] Co-transformation using a negative selectable marker gene for the production of selectable marker gene-free transgenic plants
    Jeongmoo Park
    Young Koung Lee
    Bong Kyoon Kang
    Won Il Chung
    Theoretical and Applied Genetics, 2004, 109 : 1562 - 1567
12345