TRANSFORMATION OF THE INDUSTRIAL STRAIN OF ASPERGILLUS-ORYZAE WITH THE HOMOLOGOUS AMDS GENE AS A DOMINANT SELECTABLE MARKER

被引：34
|
作者：
GOMI, K
KITAMOTO, K
KUMAGAI, C
机构：
[1] National Research Institute of Brewing, Kita-ku, Tokyo, 114, 2-6-30, Takinogawa
来源：
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1992年 / 74卷 / 06期
关键词：
D O I：
10.1016/0922-338X(92)90037-U
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A homologous transformation system has been developed for Aspergillus oryzae industrial strains using the A. oryzae acetamidase-encoding gene (amdS) as a dominant selectable marker. Although the transformation efficiency is relatively low (0.4-1 transformants per mug DNA), the transformants contained significantly high copy numbers of the plasmid integrated into the genome. In addition, the promoter of the amdS gene was replaced by the strong expression promoter of the alpha-amylase-encoding gene (amyB) from A. oryzae, thereby enhancing the selection of the transformants.
引用
收藏
页码：389 / 391
页数：3
相关论文
共 50 条
  • [21] TRANSFORMATION OF ASPERGILLUS-NIGER BY THE AMDS GENE OF ASPERGILLUS-NIDULANS
    KELLY, JM
    HYNES, MJ
    EMBO JOURNAL, 1985, 4 (02): : 475 - 479
  • [22] GROWTH-ASSOCIATED PRODUCTION OF TANNASE BY A STRAIN OF ASPERGILLUS-ORYZAE
    DOI, S
    SHINMYO, A
    ENATSU, T
    TERUI, G
    JOURNAL OF FERMENTATION TECHNOLOGY, 1973, 51 (11): : 768 - 774
  • [23] TRANSFORMATION OF PENICILLIUM-CHRYSOGENUM WITH A DOMINANT SELECTABLE MARKER
    BULL, JH
    SMITH, DJ
    TURNER, G
    CURRENT GENETICS, 1988, 13 (05) : 377 - 382
  • [24] THE DEVELOPMENT OF A HOMOLOGOUS TRANSFORMATION SYSTEM FOR ASPERGILLUS-ORYZAE BASED ON THE NITRATE ASSIMILATION PATHWAY - A CONVENIENT AND GENERAL SELECTION SYSTEM FOR FILAMENTOUS FUNGAL TRANSFORMATION
    UNKLES, SE
    CAMPBELL, EI
    DERUITERJACOBS, YMJT
    BROEKHUIJSEN, M
    MACRO, JA
    CARREZ, D
    CONTRERAS, R
    VANDENHONDEL, CAMJJ
    KINGHORN, JR
    MOLECULAR AND GENERAL GENETICS, 1989, 218 (01): : 99 - 104
  • [25] TRANSFORMATION OF MALATHION BY A FUNGUS, ASPERGILLUS-ORYZAE, ISOLATED FROM A FRESHWATER POND
    LEWIS, DL
    PARIS, DF
    BAUGHMAN, GL
    BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 1975, 13 (05) : 596 - 601
  • [26] CHARACTERIZATION OF BETA-GALACTOSIDASE FROM A SPECIAL STRAIN OF ASPERGILLUS-ORYZAE
    AKASAKI, M
    SUZUKI, M
    FUNAKOSHI, I
    YAMASHINA, I
    JOURNAL OF BIOCHEMISTRY, 1976, 80 (06): : 1195 - 1200
  • [27] RPSL(+) - A DOMINANT SELECTABLE MARKER FOR GENE REPLACEMENT IN MYCOBACTERIA
    SANDER, P
    MEIER, A
    BOTTGER, EC
    MOLECULAR MICROBIOLOGY, 1995, 16 (05) : 991 - 1000
  • [28] Gene Replacement by a Selectable Marker in the Filamentous Fungus Magnaporthe oryzae
    Garcia, Nalleli
    Farmer, Alexa N.
    Baptiste, Richmond
    Fernandez, Jessie
    BIO-PROTOCOL, 2023, 13 (17):
  • [29] EXPRESSION OF THE ALPHA-AMYLASE GENE FROM ASPERGILLUS-ORYZAE IN ASPERGILLUS-AWAMORI
    RUTTKOWSKI, E
    KHANH, NQ
    WIRSEL, S
    WILDHARDT, G
    GOTTSCHALK, M
    DECHEMA BIOTECHNOLOGY CONFERENCES, VOL 3, PTS A AND B, 1989, 3 : 325 - 328
  • [30] GENE AMPLIFICATION IN ASPERGILLUS-NIDULANS BY TRANSFORMATION WITH VECTORS CONTAINING THE AMDS GENE
    WERNARS, K
    GOOSEN, T
    WENNEKES, LMJ
    VISSER, J
    BOS, CJ
    VANDENBROEK, HWJ
    VANGORCOM, RFM
    VANDENHONDEL, CAMJJ
    POUWELS, PH
    CURRENT GENETICS, 1985, 9 (05) : 361 - 368
12345