THE ASSOCIATION OF HUMAN FIBULIN-1 WITH ELASTIC FIBERS - AN IMMUNOHISTOLOGICAL, ULTRASTRUCTURAL, AND RNA STUDY

被引:157
|
作者
ROARK, EF
KEENE, DR
HAUDENSCHILD, CC
GODYNA, S
LITTLE, CD
ARGRAVES, WS
机构
[1] AMER RED CROSS,JEROME H HOLLAND LAB,DEPT BIOCHEM,ROCKVILLE,MD 20855
[2] AMER RED CROSS,JEROME H HOLLAND LAB,DEPT EXPTL PATHOL,ROCKVILLE,MD 20855
[3] SHRINERS HOSP CRIPPLED CHILDRENS,RES UNIT,PORTLAND,OR
[4] MED UNIV S CAROLINA,DEPT CELL BIOL & ANAT,CHARLESTON,SC
关键词
EXTRACELLULAR MATRIX PROTEIN; ELASTIC FIBERS; RNA HYBRIDIZATION; REVERSE TRANSCRIPTASE PCR;
D O I
10.1177/43.4.7534784
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We examined the pattern of fibulin-1 mRNA and protein expression in human tissues and cell lines. Fibulin-1 transcripts were found in RNA isolated from most tissues and a variety of cultured cells, including fibroblasts, smooth muscle cells, and several epithelial cell lines, but not endothelial cells, lymphomyloid cells, or a number of carcinoma and melanoma lines. Immunohistochemical analysis showed that fibulin-1 is an intercellular component of connective tissues, predominantly associated with matrix fibers in tissues such as the cervix, dermis, intimal and medial layers of blood vessels, heart valves, meningeal tissue of the brain, Wharton's jelly of the umbilical cord, testis, and lung. Most of the fibers that were immunoreactive with fibulin-1 antibodies also stained with antibodies to the elastic fiber proteins elastin and fibrillin, as well as with Verhoeff's elastin stain. Immunoelectron microscopic analysis of elastin fibers of skin and saphenous vein revealed that fibulin-1 was located within the amorphous core of the fibers, similar to elastin, but it was not in the fibrillin-containing, elastin-associated microfibrils. Our finding that fibulin-1 is an elastic fiber component suggests several possible new functions for fibulin-1, e.g., that it is a structural protein that contributes to the elastic properties of connective tissue fibers or that is involved with the process of fibrogenesis.
引用
收藏
页码:401 / 411
页数:11
相关论文
共 50 条
  • [41] Serum fibulin-1 levels and target organ damage in patients at high cardiovascular risk: A prospective observational study
    Kim, Hack-Lyoung
    Lee, Jung Pyo
    Lee, Jeonghwan
    EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, 2025,
  • [42] Low level of plasma fibulin-1 in patients with thyroid lesions: a case-control study and literature review
    Mehdi Hedayati
    Raziyeh Abooshahab
    S. Adeleh Razavi
    Pouya Salehipour
    Kazem Ahmadikia
    Seti Boroomand
    Molecular Biology Reports, 2020, 47 : 8859 - 8866
  • [43] Structural and functional characterization of the human and mouse fibulin-1 gene promoters: role of Sp1 and Sp3
    Castoldi, M
    Chu, ML
    BIOCHEMICAL JOURNAL, 2002, 362 : 41 - 50
  • [44] Investigating the effect of fibulin-1 on the differentiation of human nasal inferior turbinate-derived mesenchymal stem cells into osteoblasts
    Pham, Le B. Hang
    Yoo, Yie-Ri
    Park, Sun Hwa
    Back, Sang A.
    Kim, Sung Won
    Bjorge, Isabel
    Mano, Joao
    Jang, Jun-Hyeog
    JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2017, 105 (08) : 2291 - 2298
  • [45] ULTRASTRUCTURAL CYTOCHEMISTRY OF ANCHORING FILAMENTS OF HUMAN LYMPHATIC CAPILLARIES AND THEIR RELATION TO ELASTIC FIBERS
    GERLI, R
    IBBA, L
    FRUSCHELLI, C
    LYMPHOLOGY, 1991, 24 (03) : 105 - 112
  • [46] ATTACHMENTS OF HUMAN INTRAFUSAL FIBERS (ULTRASTRUCTURAL-STUDY)
    SAHGAL, V
    SUBRAMANI, V
    MUSCLE & NERVE, 1986, 9 (05) : 226 - 226
  • [47] INTERCHROMOSOMAL FIBERS - HUMAN ULTRASTRUCTURAL STUDY BY A RECENT TECHNIQUE
    JAFFRAY, JY
    GENEIX, A
    HUMANGENETIK, 1974, 25 (02): : 119 - 126
  • [48] A BIOMARKER OF TISSUE DAMAGE, LACTATE DEHYDROGENASE, IS ASSOCIATED WITH FIBULIN-1 AND OXIDATIVE STRESS IN BLACK SOUTH AFRICANS: THE SAFREIC STUDY
    Kruger, Ruan
    Schutte, Aletta E.
    Mokwatsi, Gontse G.
    JOURNAL OF HYPERTENSION, 2016, 34 : E113 - E114
  • [49] Mitomycin C-Triggering Nuclear Translocation of Fibulin-1 Contributes to Gene Reduction in Human Corneal Fibroblasts by Microarray Analysis
    Chen, Tsan-Chi
    Chang, Shu-Wen
    INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2019, 60 (09)
  • [50] TRANS-EPITHELIAL ELIMINATION OF ELASTIC FIBERS IN KERATOACANTHOMA - AN ULTRASTRUCTURAL-STUDY
    BOWERS, D
    BHAWAN, J
    TSAY, A
    CLINICAL RESEARCH, 1989, 37 (02): : A657 - A657