THE ASSOCIATION OF HUMAN FIBULIN-1 WITH ELASTIC FIBERS - AN IMMUNOHISTOLOGICAL, ULTRASTRUCTURAL, AND RNA STUDY

被引:157
|
作者
ROARK, EF
KEENE, DR
HAUDENSCHILD, CC
GODYNA, S
LITTLE, CD
ARGRAVES, WS
机构
[1] AMER RED CROSS,JEROME H HOLLAND LAB,DEPT BIOCHEM,ROCKVILLE,MD 20855
[2] AMER RED CROSS,JEROME H HOLLAND LAB,DEPT EXPTL PATHOL,ROCKVILLE,MD 20855
[3] SHRINERS HOSP CRIPPLED CHILDRENS,RES UNIT,PORTLAND,OR
[4] MED UNIV S CAROLINA,DEPT CELL BIOL & ANAT,CHARLESTON,SC
关键词
EXTRACELLULAR MATRIX PROTEIN; ELASTIC FIBERS; RNA HYBRIDIZATION; REVERSE TRANSCRIPTASE PCR;
D O I
10.1177/43.4.7534784
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We examined the pattern of fibulin-1 mRNA and protein expression in human tissues and cell lines. Fibulin-1 transcripts were found in RNA isolated from most tissues and a variety of cultured cells, including fibroblasts, smooth muscle cells, and several epithelial cell lines, but not endothelial cells, lymphomyloid cells, or a number of carcinoma and melanoma lines. Immunohistochemical analysis showed that fibulin-1 is an intercellular component of connective tissues, predominantly associated with matrix fibers in tissues such as the cervix, dermis, intimal and medial layers of blood vessels, heart valves, meningeal tissue of the brain, Wharton's jelly of the umbilical cord, testis, and lung. Most of the fibers that were immunoreactive with fibulin-1 antibodies also stained with antibodies to the elastic fiber proteins elastin and fibrillin, as well as with Verhoeff's elastin stain. Immunoelectron microscopic analysis of elastin fibers of skin and saphenous vein revealed that fibulin-1 was located within the amorphous core of the fibers, similar to elastin, but it was not in the fibrillin-containing, elastin-associated microfibrils. Our finding that fibulin-1 is an elastic fiber component suggests several possible new functions for fibulin-1, e.g., that it is a structural protein that contributes to the elastic properties of connective tissue fibers or that is involved with the process of fibrogenesis.
引用
收藏
页码:401 / 411
页数:11
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