IDENTIFICATION OF THE PROTECTIVE 44-KILODALTON RECOMBINANT ANTIGEN OF EHRLICHIA-RISTICII

被引：1
|
作者：
SHANKARAPPA, B [1 ]
DUTTA, SK [1 ]
MATTINGLYNAPIER, B [1 ]
机构：
[1] UNIV MARYLAND, VIRGINIA MARYLAND REG COLL VET MED, COLLEGE PK, MD 20742 USA
来源：
INFECTION AND IMMUNITY | 1992年 / 60卷 / 02期
关键词：
D O I：
10.1128/IAI.60.2.612-617.1992
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Protective studies were conducted with mice by using recombinantly produced antigens, polyacrylamide gel electrophoresis-fractionated antigens, and a monoclonal antibody specific to the 28-kDa antigen of Ehrlichia risticii. Analysis of E. risticii-infected cell culture used as the challenge inoculum indicated an inverse relationship between the progression of cell culture infection and the infective capability of E. risticii for mice. A recombinant 44-kDa antigen was found to protect mice considerably against challenge infection, while the monoclonal antibody and fractionated antigens were not protective. A potentiation of protection was observed when the recombinant 44-kDa antigen was combined with the recombinant 70-kDa antigen and used for mouse immunization.
引用
收藏
页码：612 / 617
页数：6
相关论文
共 23 条
123