DIFFERENTIAL EXPRESSION OF GRANZYME-A AND GRANZYME-B PROTEASES AND THEIR SECRETION BY FRESH RAT NATURAL-KILLER-CELLS (NK) AND LYMPHOKINE-ACTIVATED KILLER-CELLS WITH NK PHENOTYPE (LAK-NK)

被引：41
|
作者：
VELOTTI, F [1 ]
PALMIERI, G [1 ]
DAMBROSIO, D [1 ]
PICCOLI, M [1 ]
FRATI, L [1 ]
SANTONI, A [1 ]
机构：
[1] CNR, INST BIOMED TECHNOL, ROME, ITALY
来源：
EUROPEAN JOURNAL OF IMMUNOLOGY | 1992年 / 22卷 / 04期
关键词：
D O I：
10.1002/eji.1830220426
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Granzymes A-G are a family of serine proteases localized in the cytoplasmic granules of cytotoxic T lymphocytes (Masson, D. et al. Cell 1987. 49: 679) and granzyme A is secreted by T lymphocytes in response to antigenic stimulation. Granzyme A is also expressed by natural killer (NK) and lymphokine-activated killer (LAK) cells. Here we show that fresh rat NK cells constitutively express granzyme B and that granzyme A and granzyme B are differentially regulated in unstimulated NK cells vs. LAK cells with NK phenotype (LAK-NK cells). We also show that both granzymes A and B are secreted in a calcium-dependent manner, by NK and LAK-NK cells in response to stimuli which trigger NK cell functions.
引用
收藏
页码：1049 / 1053
页数：5
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