PURIFICATION OF A CYTOCHROME P450 ISOZYME BELONGING TO A SUBFAMILY OF P450-IIB FROM LIVER-MICROSOMES OF GUINEA-PIGS

被引：7

作者：

NARIMATSU, S ^{[1
]}

AKUTSU, Y ^{[1
]}

MATSUNAGA, T ^{[1
]}

WATANABE, K ^{[1
]}

YAMAMOTO, I ^{[1
]}

YOSHIMURA, H ^{[1
]}

机构：

[1] KYUSHU UNIV,FAC PHARMACEUT SCI,DEPT HYG & FORENS CHEM,HIGASHI KU,FUKUOKA 812,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 172卷 / 02期

关键词：

D O I：

10.1016/0006-291X(90)90717-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An isozyme of cytochrome P450 was purified from liver microsomes of guinea pigs by HPLC with anion-exchange and hydroxylaptite columns. The isozyme showed a single band of 52 kdalton on sodium dodecyl sulfatepolyacrylamide gel electrophoresis. Oxidation activities of the final preparation towards p-nitroanisole, aniline and d-benzphetamine were 1.3 to 15.4-fold comparing with those of microsomal fraction. This isozyme was also concerned with oxidation of Δ9-tetrahydrocannabinol (THC) to 8α-hydroxy- (8α-OH-), 2′-OH- and 3′-OH-Δ9-THCs. The N-terminal region of the isozyme was considerably hydrophobic, and 13 of the first 20 amino acid residues was leucine. Since this first 20 amino acid sequence is 80% homologous with that of cytochrome P450 LM2 purified from rabbit liver microsomes, this isozyme can be categorized to a subfamily of P450 IIB. © 1990.

引用

页码：607 / 613

页数：7

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