PURIFICATION OF A CYTOCHROME P450 ISOZYME BELONGING TO A SUBFAMILY OF P450-IIB FROM LIVER-MICROSOMES OF GUINEA-PIGS

被引：7
|
作者：
NARIMATSU, S [1 ]
AKUTSU, Y [1 ]
MATSUNAGA, T [1 ]
WATANABE, K [1 ]
YAMAMOTO, I [1 ]
YOSHIMURA, H [1 ]
机构：
[1] KYUSHU UNIV,FAC PHARMACEUT SCI,DEPT HYG & FORENS CHEM,HIGASHI KU,FUKUOKA 812,JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 172卷 / 02期
关键词：
D O I：
10.1016/0006-291X(90)90717-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
An isozyme of cytochrome P450 was purified from liver microsomes of guinea pigs by HPLC with anion-exchange and hydroxylaptite columns. The isozyme showed a single band of 52 kdalton on sodium dodecyl sulfatepolyacrylamide gel electrophoresis. Oxidation activities of the final preparation towards p-nitroanisole, aniline and d-benzphetamine were 1.3 to 15.4-fold comparing with those of microsomal fraction. This isozyme was also concerned with oxidation of Δ9-tetrahydrocannabinol (THC) to 8α-hydroxy- (8α-OH-), 2′-OH- and 3′-OH-Δ9-THCs. The N-terminal region of the isozyme was considerably hydrophobic, and 13 of the first 20 amino acid residues was leucine. Since this first 20 amino acid sequence is 80% homologous with that of cytochrome P450 LM2 purified from rabbit liver microsomes, this isozyme can be categorized to a subfamily of P450 IIB. © 1990.
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收藏
页码：607 / 613
页数：7
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