KINETICS OF THE HYDROLYSIS OF L-GAMMA-GLU-PARANA BY DESTABILASE, AN ENZYME FROM THE LEECH HIRUDO-MEDICINALIS

被引：0

作者：

BASKOVA, IP

NIKONOV, GI

ZAVALOVA, LL

LARIONOVA, NI

机构：

[1] MV LOMONOSOV STATE UNIV,FAC CHEM,CHAIR CHEM ENZYMOL,MOSCOW 117234,USSR

[2] MM SHEMYAKIN BIOORGAN CHEM INST,MOSCOW 117312,USSR

来源：

BIOCHEMISTRY-MOSCOW | 1990年 / 55卷 / 04期

关键词：

DESTABILASE; ISOPEPTIDASE; L-GLUTAMIC ACID GAMMA-PARA-NITROANILIDE; MEDICINAL LEECH;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The molecular weight (12.3 kD) was determined for the enzyme destabilase, isolated from the medicinal leech Hirudo medicinalis. An investigation of the kinetics of the hydrolysis of the only synthetic substrate known at present, L-gamma-Glu-pNA,* showed that the enzyme is rather stable, withstanding 5 min heating at 70-degrees-C; the optimum pH lies in the region of 7.0-8.5. For the enzyme with specific activity 0.15.10(-9) mole/sec.mg the values K(m) = 22.10(-4) M and K(cat) = 3.53.10(-3) sec- at 37-degrees-C, pH 8.0 were determined.

引用

页码：495 / 499

页数：5

共 50 条

[41] STUDIES ON FINE-STRUCTURE OF INVERTEBRATE BLOOD-VESSELS .2. VALVES OF LATERAL SINUS OF LEECH, HIRUDO-MEDICINALIS L
HAMMERSEN, F
STAUDTE, HW
MOHRING, E
CELL AND TISSUE RESEARCH, 1976, 172 (03) : 405 - 423
[42] THE HALOMETHYLKETONE DERIVATIVE L-GLU-GAMMA-DL-ALA-CH2CL AND N-METHYL-D-ASPARTATE AS SELECTIVE ANTAGONISTS AGAINST L-GLUTAMATE AND KAINATE EXCITATION RESPECTIVELY ON RETZIUS CELLS OF THE LEECH, HIRUDO-MEDICINALIS
JAIS, AMM
SHARMA, RP
KERKUT, GA
WALKER, RJ
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-PHARMACOLOGY TOXICOLOGY & ENDOCRINOLOGY, 1984, 77 (02): : 385 - 398
[43] ISOLATION OF A COLLAGEN FRACTION FROM BODY-WALL GLYCOPROTEINS OF LEECH (HIRUDO-MEDICINALIS), AND CHARACTERIZATION OF ITS CARBOHYDRATE AMINO ACID PORTION
BISWAS, T
MUKHERJEE, AK
CARBOHYDRATE RESEARCH, 1978, 63 (JUN) : 173 - 181
[44] EGLIN-C FROM THE LEECH HIRUDO-MEDICINALIS - CHEMICAL SYNTHESIS OF THE GENE, ISOLATION AND CHARACTERIZATION OF THE POLYPEPTIDE SYNTHESIZED IN ESCHERICHIA-COLI
LIERSCH, M
RINK, H
SIEBER, P
MEYER, F
EXPERIENTIA, 1985, 41 (04): : 557 - 557
[45] Primary structure of destabilase, endo-epsilon-(gamma-glu)-lys isopeptidase from the medicinal leech
Berezhnoy, S
Tagaev, A
Zavalova, L
Baskova, I
Artamonova, I
Sverdlov, E
THROMBOSIS AND HAEMOSTASIS, 1997, : P3053 - P3053
[46] A KAZAL-TYPE INHIBITOR OF HUMAN MAST-CELL TRYPTASE - ISOLATION FROM THE MEDICAL LEECH HIRUDO-MEDICINALIS, CHARACTERIZATION, AND SEQUENCE-ANALYSIS
SOMMERHOFF, CP
SOLLNER, C
MENTELE, R
PIECHOTTKA, GP
AUERSWALD, EA
FRITZ, H
BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1994, 375 (10): : 685 - 694
[47] Genes from the medicinal leech (Hirudo medicinalis) coding for unusual enzymes that specifically cleave endo-epsilon(gamma-Glu)-Lys isopeptide bonds and help to dissolve blood clots
Zavalova, L
Lukyanov, S
Baskova, I
Snezhkov, E
Akopov, S
Berezhnoy, S
Bogdanova, E
Barsova, E
Sverdlov, ED
MOLECULAR & GENERAL GENETICS, 1996, 253 (1-2): : 20 - 25
[48] PROTON-INDUCED CHLORIDE CURRENT AND VOLTAGE-ACTIVATED NA+ AND CA2+ CURRENTS IN EMBRYONIC NEURONS FROM THE MEDICINAL LEECH (HIRUDO-MEDICINALIS)
GOTTMANN, K
DIETZEL, ID
LUX, HD
NEUROSCIENCE LETTERS, 1989, 96 (02) : 173 - 178
[49] THE PRIMARY STRUCTURE OF BDELLIN B-3 FROM THE LEECH HIRUDO-MEDICINALIS - BDELLIN B-3 IS A COMPACT PROTEINASE-INHIBITOR OF A NONCLASSICAL KAZAL TYPE - IT IS PRESENT IN THE LEECH IN A HIGH MOLECULAR MASS FORM
FINK, E
REHM, H
GIPPNER, C
BODE, W
EULITZ, M
MACHLEIDT, W
FRITZ, H
BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1986, 367 (12): : 1235 - 1242
[50] A LARGE FRAGMENT APPROACH TO DNA-SYNTHESIS - TOTAL SYNTHESIS OF A GENE FOR THE PROTEASE INHIBITOR EGLIN-C FROM THE LEECH HIRUDO-MEDICINALIS AND ITS EXPRESSION IN ESCHERICHIA-COLI
RINK, H
LIERSCH, M
SIEBER, P
MEYER, F
NUCLEIC ACIDS RESEARCH, 1984, 12 (16) : 6369 - 6387

← 1 2 3 4 5 →