KINETICS OF THE HYDROLYSIS OF L-GAMMA-GLU-PARANA BY DESTABILASE, AN ENZYME FROM THE LEECH HIRUDO-MEDICINALIS

被引：0
|
作者：
BASKOVA, IP
NIKONOV, GI
ZAVALOVA, LL
LARIONOVA, NI
机构：
[1] MV LOMONOSOV STATE UNIV,FAC CHEM,CHAIR CHEM ENZYMOL,MOSCOW 117234,USSR
[2] MM SHEMYAKIN BIOORGAN CHEM INST,MOSCOW 117312,USSR
来源：
BIOCHEMISTRY-MOSCOW | 1990年 / 55卷 / 04期
关键词：
DESTABILASE; ISOPEPTIDASE; L-GLUTAMIC ACID GAMMA-PARA-NITROANILIDE; MEDICINAL LEECH;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The molecular weight (12.3 kD) was determined for the enzyme destabilase, isolated from the medicinal leech Hirudo medicinalis. An investigation of the kinetics of the hydrolysis of the only synthetic substrate known at present, L-gamma-Glu-pNA,* showed that the enzyme is rather stable, withstanding 5 min heating at 70-degrees-C; the optimum pH lies in the region of 7.0-8.5. For the enzyme with specific activity 0.15.10(-9) mole/sec.mg the values K(m) = 22.10(-4) M and K(cat) = 3.53.10(-3) sec- at 37-degrees-C, pH 8.0 were determined.
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页码：495 / 499
页数：5
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