Crystallization of the apo-forms of the D-lactate dehydrogenases from Lactobacillus pentosum and Leuconostoc mesenteroides

被引：0
|
作者：
Stoll, VS
Kimber, MS
Macfarlane, LA
Eikmanns, U
Taguchi, H
Pai, EF
机构：
[1] UNIV TORONTO,DEPT BIOCHEM,TORONTO,ON M5S 1A8,CANADA
[2] UNIV TORONTO,DEPT MED & MOLEC GENET,TORONTO,ON M5S 1A8,CANADA
[3] UNIV TORONTO,PROT ENGN NETWORK CTR EXCELLENCE,TORONTO,ON M5S 1A8,CANADA
[4] SCI UNIV TOKYO,FAC SCI & TECHNOL,DEPT APPL BIOL SCI,NODA,CHIBA 278,JAPAN
来源：
PROTEIN AND PEPTIDE LETTERS | 1995年 / 2卷 / 03期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
NADH dependent D-lactate dehydrogenases from Lactobacillus pentosum and from Leuconostoc mesenteroides have been crystallized in their ape-forms by the hanging drop method. The L. pentosum enzyme adopts space group pi with unit cell dimensions a = 54.5 Angstrom, b = 58.2 Angstrom, c = 61.2 Angstrom, alpha = 74.4 degrees, beta = 83.9 degrees and gamma = 82.9 degrees. The L. mesenteriodes enzyme crystallizes in space group C2 with a unit cell of a = 223.2 Angstrom, b = 62.8 Angstrom, c = 143.5 Angstrom, beta = 92.4 degrees. The crystals diffract to at least 2.4 Angstrom and 3.0 Angstrom resolution, respectively, and are stable in the x-ray beam.
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页码：435 / 440
页数：6
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