SYNTHESIS OF 3,4-CIS-[H-3]LEUCOCYANIDIN AND ENZYMATIC REDUCTION TO CATECHIN

被引：38
|
作者：
TANNER, GJ [1 ]
KRISTIANSEN, KN [1 ]
机构：
[1] CARLSBERG LAB,DEPT PHYSIOL,DK-2500 COPENHAGEN,DENMARK
来源：
ANALYTICAL BIOCHEMISTRY | 1993年 / 209卷 / 02期
关键词：
D O I：
10.1006/abio.1993.1119
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A novel method is presented for the synthesis and purification of (+)-2,3-trans-3,4-cis-[4-3H]leucocyanidin. Soluble enzyme extracts from developing barley grains and leaves of the forage legume Onobrychis viciifolia (sainfoin) catalyzed the NAD(P)H-dependent reduction of (+)-2,3-trans-3,4-cis-[4-3H]leucocyanidin to (+)-]43H]catechin. NADPH was the preferred substrate. With extracts of barley the rate of reaction with 1 mM NADH was 20% of the rate found with NADPH. With extracts from both tissues there was a broad pH optimum around pH 6.6. © 1994 Academic Press, Inc. All rights reserved.
引用
收藏
页码：274 / 277
页数：4
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