SYNTHESIS OF 3,4-CIS-[H-3]LEUCOCYANIDIN AND ENZYMATIC REDUCTION TO CATECHIN

被引：38

作者：

TANNER, GJ ^{[1
]}

KRISTIANSEN, KN ^{[1
]}

机构：

[1] CARLSBERG LAB,DEPT PHYSIOL,DK-2500 COPENHAGEN,DENMARK

来源：

ANALYTICAL BIOCHEMISTRY | 1993年 / 209卷 / 02期

关键词：

D O I：

10.1006/abio.1993.1119

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel method is presented for the synthesis and purification of (+)-2,3-trans-3,4-cis-[4-3H]leucocyanidin. Soluble enzyme extracts from developing barley grains and leaves of the forage legume Onobrychis viciifolia (sainfoin) catalyzed the NAD(P)H-dependent reduction of (+)-2,3-trans-3,4-cis-[4-3H]leucocyanidin to (+)-]43H]catechin. NADPH was the preferred substrate. With extracts of barley the rate of reaction with 1 mM NADH was 20% of the rate found with NADPH. With extracts from both tissues there was a broad pH optimum around pH 6.6. © 1994 Academic Press, Inc. All rights reserved.

引用

页码：274 / 277

页数：4

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