ISOLATION OF A REPEATED SEQUENCE FROM THE GENOME OF BORDETELLA-PARAPERTUSSIS WITH CHARACTERISTICS OF AN INSERTION-SEQUENCE ELEMENT

被引：0

作者：

VANDERZEE, A ^{[1
]}

AGTERBERG, C ^{[1
]}

VANAGTERVELD, M ^{[1
]}

PEETERS, M ^{[1
]}

MOOI, F ^{[1
]}

机构：

[1] INST PUBL HLTH & ENVIRONM PROTECT,BILTHOVEN,NETHERLANDS

来源：

ZENTRALBLATT FUR BAKTERIOLOGIE-INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY VIROLOGY PARASITOLOGY AND INFECTIOUS DISEASES | 1993年 / 278卷 / 2-3期

关键词：

D O I：

10.1016/S0934-8840(11)80862-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A repeated DNA sequence in the genome of Bordetella parapertussis was identified by means of heteroduplex formation and Sl nuclease treatment. Cloning and sequencing of the repeated sequence, revealed that it comprised 1306 basepairs, contained inverted repeats at its termini, and two large open reading frames. This insertion sequence element was designated IS1001, and was found in about 20 copies in 10 B. parapertussis strains analyzed. Identical hybridization patterns were observed in B. parapertussis when IS1 001 was used as a probe. B. pertussis strains did not hybridize to IS1001.

引用

页码：451 / 455

页数：5

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