A repeated DNA sequence in the genome of Bordetella parapertussis was identified by means of heteroduplex formation and Sl nuclease treatment. Cloning and sequencing of the repeated sequence, revealed that it comprised 1306 basepairs, contained inverted repeats at its termini, and two large open reading frames. This insertion sequence element was designated IS1001, and was found in about 20 copies in 10 B. parapertussis strains analyzed. Identical hybridization patterns were observed in B. parapertussis when IS1 001 was used as a probe. B. pertussis strains did not hybridize to IS1001.
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Inst Oswaldo Cruz, FIOCRUZ, Lab Genet Mol Microorganismos, BR-20001 Rio De Janeiro, RJ, BrazilInst Oswaldo Cruz, FIOCRUZ, Lab Genet Mol Microorganismos, BR-20001 Rio De Janeiro, RJ, Brazil
Andrade, Bruno Gabriel N.
Abanto Marin, Michel F.
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Inst Oswaldo Cruz, FIOCRUZ, Lab Genet Mol Microorganismos, BR-20001 Rio De Janeiro, RJ, BrazilInst Oswaldo Cruz, FIOCRUZ, Lab Genet Mol Microorganismos, BR-20001 Rio De Janeiro, RJ, Brazil
Abanto Marin, Michel F.
Cambuy, Diego Duque
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Inst Oswaldo Cruz, FIOCRUZ, Lab Genet Mol Microorganismos, BR-20001 Rio De Janeiro, RJ, BrazilInst Oswaldo Cruz, FIOCRUZ, Lab Genet Mol Microorganismos, BR-20001 Rio De Janeiro, RJ, Brazil
Cambuy, Diego Duque
Fonseca, Erica Lourenco
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Inst Oswaldo Cruz, FIOCRUZ, Lab Genet Mol Microorganismos, BR-20001 Rio De Janeiro, RJ, BrazilInst Oswaldo Cruz, FIOCRUZ, Lab Genet Mol Microorganismos, BR-20001 Rio De Janeiro, RJ, Brazil
Fonseca, Erica Lourenco
Souza, Nadjla Ferreira
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Lab Cent Saude Publ Dr Milton Sobral, Recife, PE, BrazilInst Oswaldo Cruz, FIOCRUZ, Lab Genet Mol Microorganismos, BR-20001 Rio De Janeiro, RJ, Brazil
Souza, Nadjla Ferreira
Vicente, Ana Carolina P.
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Inst Oswaldo Cruz, FIOCRUZ, Lab Genet Mol Microorganismos, BR-20001 Rio De Janeiro, RJ, BrazilInst Oswaldo Cruz, FIOCRUZ, Lab Genet Mol Microorganismos, BR-20001 Rio De Janeiro, RJ, Brazil
Vicente, Ana Carolina P.
MEMORIAS DO INSTITUTO OSWALDO CRUZ,
2014,
109
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: 952
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