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SURFACE-ACOUSTIC-WAVE SENSOR SYSTEM APPLIED TO THE KINETIC-STUDY OF UREASE IN PLANT SEEDS
被引:0
|作者:
LIU, DZ
[1
]
CHEN, K
[1
]
GE, K
[1
]
NIE, LH
[1
]
YAO, SZ
[1
]
机构:
[1] HUNAN UNIV,NEW MAT RES INST,CHANGSHA 410082,PEOPLES R CHINA
关键词:
SURFACE ACOUSTIC WAVE;
SENSOR;
UREASE;
UREA;
KINETIC PARAMETERS;
D O I:
暂无
中图分类号:
O6 [化学];
学科分类号:
0703 ;
摘要:
A new type of the surface acoustic wave (SAW) sensor system was delivered. Urease from several kinds of plant seeds was extracted with different extracting solvents. The urease activity, Michaelis constant and other kinetic parameters were estimated for the first time by means of the new device-SAW sensor system. Some factors such as pH, temperature, activators and inhibitors are also discussed. The method can be applied to the determination of urea content in human urine and the experimental results consist with those reported.
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页码:231 / 240
页数:10
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