BOMBESIN STIMULATES C-FOS AND C-JUN MESSENGER-RNAS IN SMALL-CELL LUNG-CANCER CELLS

被引：42
|
作者：
DRAOUI, M
CHUNG, P
PARK, M
BIRRER, M
JAKOWLEW, S
MOODY, TW
机构：
[1] NCI,DCPC,BIOMARKERS & PREVENT RES BRANCH,ROCKVILLE,MD 20850
[2] GEORGE WASHINGTON UNIV,MED CTR,DEPT MOLEC BIOL & BIOCHEM,WASHINGTON,DC 20037
来源：
PEPTIDES | 1995年 / 16卷 / 02期
关键词：
SMALL CELL LUNG CANCER (SCLC); GRP RECEPTOR; C-FOS; C-JUN;
D O I：
10.1016/0196-9781(94)00173-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effects of bombesin/gastrin-releasing peptide (BN/GRP) on c-fos and c-jun gene expression were investigated using small cell lung cancer (SCLC) cells. BN(10 nM) increased c-fos mRNA fivefold using NCI-H345 or NCI-HS 10 cells. The increase was concentration dependent with 1 nM BN half-maximally increasing c-fos mRNA. Also, the increase in c-fos mRNA caused by BN was time dependent, being maximal after 1 h and returning to basal values after 4 h. GRP and GRP(14-27) but not GRP(1-16) increased c-fos mRNA. BW2258U89 (1 mu M), a GRP receptor antagonist, had no effect on basal c-fos but inhibited the increase in c-fos mRNA caused by 10 nM BN. Also, BN transiently increased c-jun mRNA twofold and the increase caused by BN was blocked by BW2258U89. These data suggest that GRP receptors may regulate nuclear oncogene gene expression in SCLC cells.
引用
收藏
页码：289 / 292
页数：4
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