BOMBESIN STIMULATES C-FOS AND C-JUN MESSENGER-RNAS IN SMALL-CELL LUNG-CANCER CELLS

被引：42
|
作者：
DRAOUI, M
CHUNG, P
PARK, M
BIRRER, M
JAKOWLEW, S
MOODY, TW
机构：
[1] NCI,DCPC,BIOMARKERS & PREVENT RES BRANCH,ROCKVILLE,MD 20850
[2] GEORGE WASHINGTON UNIV,MED CTR,DEPT MOLEC BIOL & BIOCHEM,WASHINGTON,DC 20037
来源：
PEPTIDES | 1995年 / 16卷 / 02期
关键词：
SMALL CELL LUNG CANCER (SCLC); GRP RECEPTOR; C-FOS; C-JUN;
D O I：
10.1016/0196-9781(94)00173-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effects of bombesin/gastrin-releasing peptide (BN/GRP) on c-fos and c-jun gene expression were investigated using small cell lung cancer (SCLC) cells. BN(10 nM) increased c-fos mRNA fivefold using NCI-H345 or NCI-HS 10 cells. The increase was concentration dependent with 1 nM BN half-maximally increasing c-fos mRNA. Also, the increase in c-fos mRNA caused by BN was time dependent, being maximal after 1 h and returning to basal values after 4 h. GRP and GRP(14-27) but not GRP(1-16) increased c-fos mRNA. BW2258U89 (1 mu M), a GRP receptor antagonist, had no effect on basal c-fos but inhibited the increase in c-fos mRNA caused by 10 nM BN. Also, BN transiently increased c-jun mRNA twofold and the increase caused by BN was blocked by BW2258U89. These data suggest that GRP receptors may regulate nuclear oncogene gene expression in SCLC cells.
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页码：289 / 292
页数：4
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