MOLECULAR-CLONING AND CHARACTERIZATION OF CDNA FOR THE PRECURSOR OF RAT PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP)

被引：244

作者：

OGI, K

KIMURA, C

ONDA, H

ARIMURA, A

FUJINO, M

机构：

[1] TAKEDA CHEM IND LTD,TSUKUBA RES LABS,7 WADAI,TSUKUBA,IBARAKI 30042,JAPAN

[2] TULANE UNIV,HEBERT CTR,US JAPAN BIOMED RES LABS,BELLE CHASSE,LA 70037

[3] TULANE UNIV,SCH MED,DEPT MED,NEW ORLEANS,LA 70121

[4] TULANE UNIV,SCH MED,DEPT PHYSIOL & ANAT,NEW ORLEANS,LA 70121

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 173卷 / 03期

关键词：

D O I：

10.1016/S0006-291X(05)80924-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated and cloned a cDNA for rat pituitary adenylate cyclase activating polypeptide (PACAP) precursor protein from the brain. The nucleotide and the deduced amino acid sequences revealed that the rat PACAP cDNA encoded a 175 amino acid protein highly homologous to the ovine and human PACAP precursors. The rat PACAP precursor protein included a putative signal peptide at the amino-terminus, a PACAP-related peptide (PRP) of 29 amino acid residues and the rat PACAP of 38 amino acid residues flanked by basic processing sites, and a carboxy-teminus amidation signal for PACAP. Furthermore, the amino acid sequence of the rat PACAP was completely identical to that of the ovine and human PACAP. Northern blot analysis of rat brain RNA revealed an approximately 3.0 kb transcript for the PACAP precursor in rat brain. © 1990 Academic Press, Inc.

引用

页码：1271 / 1279

页数：9

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