MOLECULAR-CLONING AND CHARACTERIZATION OF CDNA FOR THE PRECURSOR OF RAT PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP)

被引：244
|
作者：
OGI, K
KIMURA, C
ONDA, H
ARIMURA, A
FUJINO, M
机构：
[1] TAKEDA CHEM IND LTD,TSUKUBA RES LABS,7 WADAI,TSUKUBA,IBARAKI 30042,JAPAN
[2] TULANE UNIV,HEBERT CTR,US JAPAN BIOMED RES LABS,BELLE CHASSE,LA 70037
[3] TULANE UNIV,SCH MED,DEPT MED,NEW ORLEANS,LA 70121
[4] TULANE UNIV,SCH MED,DEPT PHYSIOL & ANAT,NEW ORLEANS,LA 70121
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 173卷 / 03期
关键词：
D O I：
10.1016/S0006-291X(05)80924-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have isolated and cloned a cDNA for rat pituitary adenylate cyclase activating polypeptide (PACAP) precursor protein from the brain. The nucleotide and the deduced amino acid sequences revealed that the rat PACAP cDNA encoded a 175 amino acid protein highly homologous to the ovine and human PACAP precursors. The rat PACAP precursor protein included a putative signal peptide at the amino-terminus, a PACAP-related peptide (PRP) of 29 amino acid residues and the rat PACAP of 38 amino acid residues flanked by basic processing sites, and a carboxy-teminus amidation signal for PACAP. Furthermore, the amino acid sequence of the rat PACAP was completely identical to that of the ovine and human PACAP. Northern blot analysis of rat brain RNA revealed an approximately 3.0 kb transcript for the PACAP precursor in rat brain. © 1990 Academic Press, Inc.
引用
收藏
页码：1271 / 1279
页数：9
相关论文
共 50 条
  • [31] DEMONSTRATION OF SPECIFIC BINDING-SITES FOR PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP) IN RAT ASTROCYTES
    TATSUNO, I
    GOTTSCHALL, PE
    KOVES, K
    ARIMURA, A
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1990, 168 (03) : 1027 - 1033
  • [32] ANTINOCICEPTIVE EFFECT OF INTRATHECALLY ADMINISTERED PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP) ON THE RAT FORMALIN TEST
    YAMAMOTO, T
    TATSUNO, I
    [J]. NEUROSCIENCE LETTERS, 1995, 184 (01) : 32 - 35
  • [33] PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP) REDUCES FOOD-INTAKE IN MICE
    MORLEY, JE
    HOROWITZ, M
    MORLEY, PMK
    FLOOD, JF
    [J]. PEPTIDES, 1992, 13 (06) : 1133 - 1135
  • [34] EFFECTS OF PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP) ON THE DC ERG OF THE RABBIT EYE
    JARKMAN, S
    KATO, M
    BRAGADOTTIR, R
    NILSSON, SEG
    [J]. VISION RESEARCH, 1995, 35 : P430 - P430
  • [35] cDNA cloning of a mouse pituitary adenylate cyclase-activating polypeptide receptor
    Hashimoto, H
    Yamamoto, K
    Hagihara, N
    Ogawa, N
    Nishino, A
    Aino, H
    Nogi, H
    Imanishi, K
    Matsuda, T
    Baba, A
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1996, 1281 (02): : 129 - 133
  • [36] PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP) AND ITS RECEPTORS - NEUROENDOCRINE AND ENDOCRINE INTERACTION
    ARIMURA, A
    SHIODA, S
    [J]. FRONTIERS IN NEUROENDOCRINOLOGY, 1995, 16 (01) : 53 - 88
  • [37] cDNA cloning of a mouse pituitary adenylate cyclase-activating polypeptide receptor
    [J]. B B A Biomembr, 2 (129):
  • [38] VASODILATORY EFFECT OF PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP) AND ITS RELATED PEPTIDES
    NARUSE, S
    OZAKI, T
    NOKIHARA, K
    [J]. REGULATORY PEPTIDES, 1992, 40 (02) : 214 - 214
  • [39] DINUCLEOTIDE REPEAT POLYMORPHISM AT THE HUMAN PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP) GENE
    PEREZJURADO, LA
    FRANCKE, U
    [J]. HUMAN MOLECULAR GENETICS, 1993, 2 (06) : 827 - 827
  • [40] EFFECT OF PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE (PACAP) ON 7B2 RELEASE IN RAT PITUITARY AND HYPOTHALAMUS
    GABB, S
    SUZUKI, Y
    GHATEI, MA
    BLOOM, SR
    [J]. REGULATORY PEPTIDES, 1990, 30 (01) : 43 - 43
12345