CLEAVAGE OF RNA BY HYBRIDASE .5. COMPLEMENTATION FIDELITY OF CLEAVAGE

被引：0

作者：

METELEV, VG ^{[1
]}

KRYNETSKAYA, NF ^{[1
]}

VOLKOV, EM ^{[1
]}

ORETSKAYA, TS ^{[1
]}

SHABAROVA, ZA ^{[1
]}

机构：

[1] MV LOMONOSOV STATE UNIV,FAC CHEM,MOSCOW 119899,USSR

来源：

MOLECULAR BIOLOGY | 1991年 / 25卷 / 06期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Studies were carried out on the efficiency of hybridase cleavage of RNA in perfect and imperfect hybrid duplexes consisting of three 'components: 1) individual RNA or polyribonucleotides; 2) synthetic complementary oligodeoxyribonucleotides; 3) Escherichia coli RNase H. In most experiments, the RNA was hydrolyzed predominantly in perfect hybrid duplexes formed from the RNA target and the complementary oligonucleotide probes. A number of imperfect hybrid duplexes containing a central pair of noncomplementary bases were found in which efficient hybridase cleavage of RNA also occurred.

引用

页码：1240 / 1246

页数：7

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