INDUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIIDIOTYPIC ANTIBODIES TO TICK-BORNE ENCEPHALITIS-VIRUS NEUTRALIZING ANTIBODY

被引：1
|
作者：
KOPECKY, J [1 ]
KREJCI, R [1 ]
GOULD, EA [1 ]
机构：
[1] NERC,INST VIROL,OXFORD OX1 3SR,ENGLAND
来源：
JOURNAL OF IMMUNOASSAY | 1995年 / 16卷 / 04期
关键词：
ANTIIDIOTYPIC ANTIBODIES; TICK-BORNE ENCEPHALITIS VIRUS;
D O I：
10.1080/15321819508013572
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Seven monoclonal anti-idiotypic antibodies (anti-ID MAbs, Ab2) were generated against virus-neutralizing ana hemagglutination-inhibiting monoclonal antibody (Ab1) specific for tick-borne encephalitis (TEE) virus. Six of these anti-ID MAbs inhibited the binding of Abl to the virus antigen, thus classifying these anti-ID antibodies as Ab2 beta or AB2 gamma. Inhibition tests with heterologous anti-TBE sera revealed that these anti-ID MAbs were not recognized by anti-TEE antibodies and therefore they do not carry an internal image of TEE virus antigen. Hence, the anti-ID MAbs may be classified as Ab2 gamma type. None of the anti-1D MAbs induced production of antiviral antibodies nor protective immunity in syngeneic Balb/c mice. Using these anti-ID MAbs four nonoverlapping idiotopes were identified on Ab1 variable region.
引用
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页码：437 / 465
页数：29
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