INDUCTION AND CHARACTERIZATION OF MONOCLONAL ANTIIDIOTYPIC ANTIBODIES TO TICK-BORNE ENCEPHALITIS-VIRUS NEUTRALIZING ANTIBODY

被引：1
|
作者：
KOPECKY, J [1 ]
KREJCI, R [1 ]
GOULD, EA [1 ]
机构：
[1] NERC,INST VIROL,OXFORD OX1 3SR,ENGLAND
来源：
JOURNAL OF IMMUNOASSAY | 1995年 / 16卷 / 04期
关键词：
ANTIIDIOTYPIC ANTIBODIES; TICK-BORNE ENCEPHALITIS VIRUS;
D O I：
10.1080/15321819508013572
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Seven monoclonal anti-idiotypic antibodies (anti-ID MAbs, Ab2) were generated against virus-neutralizing ana hemagglutination-inhibiting monoclonal antibody (Ab1) specific for tick-borne encephalitis (TEE) virus. Six of these anti-ID MAbs inhibited the binding of Abl to the virus antigen, thus classifying these anti-ID antibodies as Ab2 beta or AB2 gamma. Inhibition tests with heterologous anti-TBE sera revealed that these anti-ID MAbs were not recognized by anti-TEE antibodies and therefore they do not carry an internal image of TEE virus antigen. Hence, the anti-ID MAbs may be classified as Ab2 gamma type. None of the anti-1D MAbs induced production of antiviral antibodies nor protective immunity in syngeneic Balb/c mice. Using these anti-ID MAbs four nonoverlapping idiotopes were identified on Ab1 variable region.
引用
收藏
页码：437 / 465
页数：29
相关论文
共 50 条
  • [31] THE MOLECULAR-BIOLOGY OF TICK-BORNE ENCEPHALITIS-VIRUS
    HEINZ, FX
    MANDL, CW
    APMIS, 1993, 101 (10) : 735 - 745
  • [32] SLOWLY SEDIMENTING HEMAGGLUTININ OF TICK-BORNE ENCEPHALITIS-VIRUS
    GRITSUN, TS
    LISAK, VM
    LYAPUSTIN, VN
    KOROLEV, MB
    LASHKEVICH, VA
    VOPROSY VIRUSOLOGII, 1989, (04) : 449 - 454
  • [33] NONVIRION (SOLUBLE) ANTIGEN OF TICK-BORNE ENCEPHALITIS-VIRUS
    GRITSUN, TS
    LYAPUSTIN, VN
    KARGANOVA, GG
    LASHKEVICH, VA
    VOPROSY VIRUSOLOGII, 1988, (02) : 217 - 227
  • [34] PERSISTENCE OF TICK-BORNE ENCEPHALITIS-VIRUS AND ITS SEQUELS
    POGODINA, VV
    VESTNIK AKADEMII MEDITSINSKIKH NAUK SSSR, 1983, (05): : 67 - 73
  • [35] MECHANISM OF TICK-BORNE ENCEPHALITIS-VIRUS MUTAGENIC EFFECT
    GORDEEVA, NI
    VOPROSY VIRUSOLOGII, 1979, (02) : 161 - 165
  • [36] CHARACTERISTICS OF TICK-BORNE ENCEPHALITIS-VIRUS CIRCULATING IN POLAND
    NAWROCKA, E
    ACTA MICROBIOLOGICA POLONICA SERIES A-MICROBIOLOGIA GENERALIS, 1975, 7 (04): : 237 - 245
  • [37] TICK-BORNE ENCEPHALITIS-VIRUS ACTIVITY IN STYRIA, AUSTRIA
    LABUDA, M
    STUNZNER, D
    KOZUCH, O
    SIXL, W
    KOCIANOVA, E
    SCHAFFLER, R
    VYROSTEKOVA, V
    ACTA VIROLOGICA, 1993, 37 (2-3) : 187 - 190
  • [38] ANTIBODY STATUS OF FORESTRY WORKERS VACCINATED AGAINST TICK-BORNE ENCEPHALITIS-VIRUS
    FERENCZI, E
    ACTA MICROBIOLOGICA HUNGARICA, 1984, 31 (03): : 275 - 275
  • [39] Neutralizing single-chain antibodies against the tick-borne encephalitis virus
    L. N. Levanov
    L. E. Matveev
    T. E. Yun
    E. P. Goncharova
    L. R. Lebedev
    A. N. Shvalov
    A. B. Ryzhykov
    I. K. Baikov
    V. A. Matveeva
    V. A. Rikhter
    N. V. Tikunova
    Russian Journal of Bioorganic Chemistry, 2009, 35 : 474 - 481
  • [40] Neutralizing Single-Chain Antibodies Against the Tick-Borne Encephalitis Virus
    Levanov, L. N.
    Matveev, L. E.
    Yun, T. E.
    Goncharova, E. P.
    Lebedev, L. R.
    Shvalov, A. N.
    Ryzhykov, A. B.
    Baikov, I. K.
    Matveeva, V. A.
    Rikhter, V. A.
    Tikunova, N. V.
    RUSSIAN JOURNAL OF BIOORGANIC CHEMISTRY, 2009, 35 (04) : 474 - 481
12345