MICROBIOLOGICAL ASSAY FOR VITAMIN-B-12 PERFORMED IN 96-WELL MICROTITRE PLATES

被引：244
|
作者：
KELLEHER, BP [1 ]
BROIN, SDO [1 ]
机构：
[1] ST JAMES HOSP,DEPT HAEMATOL,JAMES ST,DUBLIN 8,IRELAND
来源：
JOURNAL OF CLINICAL PATHOLOGY | 1991年 / 44卷 / 07期
关键词：
D O I：
10.1136/jcp.44.7.592
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
A simplified microbiological assay for vitamin B12 estimation, completed on microtitre plates using a colistin sulphate resistant strain of Lactobacillus leichmannii (NCIB 12519), and cryopreserved cultures is described. The new assay correlated well with a more conventional "tube" assay and was not influenced by the presence of antibiotics in serum. Evaluation of assay performance showed excellent interassay and intra-assay precision with quantitative recovery of added cyanocobalamin over a wide range of additions (94.9%-102.1%). The advantages of short incubation time, easy reading, and minimal reagent costs make this assay an attractive option in the routine clinical laboratory and in research.
引用
收藏
页码：592 / 595
页数：4
相关论文
共 50 条
  • [41] A solid-phase Bcr-Abl kinase assay in 96-well hydrogel plates
    Wu, Ding
    Mand, Michael R.
    Veach, Darren R.
    Parker, Laurie L.
    Clarkson, Bayard.
    Kron, Stephen J.
    ANALYTICAL BIOCHEMISTRY, 2008, 375 (01) : 18 - 26
  • [42] A NOTE ON THE MICROBIOLOGICAL ASSAY FOR VITAMIN-B12
    LOY, HW
    KLINE, OL
    JOURNAL OF THE ASSOCIATION OF OFFICIAL AGRICULTURAL CHEMISTS, 1951, 34 (01): : 225 - 226
  • [43] CHEMILUMINESCENCE RECEPTOR ASSAY FOR MEASURING VITAMIN-B-12 IN SERUM EVALUATED
    WENTWORTH, S
    MCBRIDE, JA
    WALKER, WHC
    CLINICAL CHEMISTRY, 1994, 40 (04) : 537 - 540
  • [44] SCREENING MUTANTS BY A MODIFIED SANGER DIDEOXY SEQUENCING METHOD IN 96-WELL MICROTITRE TRAYS
    BANDEA, CI
    YANG, NQ
    WU, GJ
    BIOTECHNIQUES, 1989, 7 (02) : 142 - 143
  • [45] A microfabricated 96-well wound-healing assay
    Luan, Shaoliang
    Hao, Rui
    Wei, Yuanchen
    Chen, Deyong
    Fan, Beiyuan
    Dong, Fengliang
    Guo, Wei
    Wang, Junbo
    Chen, Jian
    CYTOMETRY PART A, 2017, 91A (12) : 1192 - 1199
  • [46] 96-well plate assay for sublethal metabolic activity
    Yang, YS
    Balcarcel, RR
    ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES, 2004, 2 (04) : 353 - 361
  • [47] A 96-well assay for uronic acid carbazole reaction
    Cesaretti, M
    Luppi, E
    Maccari, F
    Volpi, N
    CARBOHYDRATE POLYMERS, 2003, 54 (01) : 59 - 61
  • [48] MICROBIOLOGICAL ASSAY OF VITAMIN-B12 AND PSEUDOVITAMIN-B12
    BERMAN, D
    YACOWITZ, H
    WEISER, HH
    POULTRY SCIENCE, 1954, 33 (05) : 1043 - 1043
  • [49] Luminometric assay of platelet activation in 96-well microplate
    Sun, B
    Tandon, NN
    Yamamoto, N
    Yoshitake, M
    Kambayashi, J
    BIOTECHNIQUES, 2001, 31 (05) : 1174 - +
  • [50] MONO-SUBSTITUTED VITAMIN-B-12 AMIDES .1. A MICROBIOLOGICAL STUDY
    BAKER, H
    FRANK, O
    PASHER, I
    HUTNER, SH
    HERBERT, V
    SOBOTKA, H
    PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE, 1959, 100 (04): : 825 - 827
12345