MICROBIOLOGICAL ASSAY FOR VITAMIN-B-12 PERFORMED IN 96-WELL MICROTITRE PLATES

被引：244
|
作者：
KELLEHER, BP [1 ]
BROIN, SDO [1 ]
机构：
[1] ST JAMES HOSP,DEPT HAEMATOL,JAMES ST,DUBLIN 8,IRELAND
来源：
JOURNAL OF CLINICAL PATHOLOGY | 1991年 / 44卷 / 07期
关键词：
D O I：
10.1136/jcp.44.7.592
中图分类号：
R36 [病理学];
学科分类号：
100104 ;
摘要：
A simplified microbiological assay for vitamin B12 estimation, completed on microtitre plates using a colistin sulphate resistant strain of Lactobacillus leichmannii (NCIB 12519), and cryopreserved cultures is described. The new assay correlated well with a more conventional "tube" assay and was not influenced by the presence of antibiotics in serum. Evaluation of assay performance showed excellent interassay and intra-assay precision with quantitative recovery of added cyanocobalamin over a wide range of additions (94.9%-102.1%). The advantages of short incubation time, easy reading, and minimal reagent costs make this assay an attractive option in the routine clinical laboratory and in research.
引用
收藏
页码：592 / 595
页数：4
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