VALIDATION OF A HPLC METHOD FOR THE DETERMINATION OF PROPYLTHIOURACIL IN PLASMA

被引：8

作者：

KABANDA, L ^{[1
]}

DEMUYNCK, C ^{[1
]}

LEFEBVRE, RA ^{[1
]}

REMON, JP ^{[1
]}

机构：

[1] STATE UNIV GHENT,SCH MED,HEYMANS INST PHARMACOL,B-9000 GHENT,BELGIUM

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1994年 / 17卷 / 09期

关键词：

D O I：

10.1080/10826079408013480

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A high-performance liquid chromatography method for the analysis of propylthiouracil in plasma was validated. Methylthiouracil was used as the internal standard. A one step extraction procedure and an isocratic HPLC method with UV detection were used. No plasma components were found to interfere in the assay. Linear calibration (r(2) > 0.99) curves using water and plasma as matrices in the range of 0.05 to 15 mu g.mL(-1) were obtained. Good recoveries for propylthiouracil (> 85%) and methylthiouracil (> 65%) were seen both in water and in plasma. The coefficient of variation for repeatability was < 7%, for reproducibility < 8% and for accuracy < 6%. The limit of detection was 2 ng.mL(-1) in water and 5 ng.mL(-1) in plasma. A poor stability of propylthiouracil was observed even at -20 degrees C and it is recommended to perform the analyze shortly after sampling.

引用

页码：2069 / 2083

页数：15

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