VALIDATION OF A HPLC METHOD FOR THE DETERMINATION OF PROPYLTHIOURACIL IN PLASMA

被引：8
|
作者：
KABANDA, L [1 ]
DEMUYNCK, C [1 ]
LEFEBVRE, RA [1 ]
REMON, JP [1 ]
机构：
[1] STATE UNIV GHENT,SCH MED,HEYMANS INST PHARMACOL,B-9000 GHENT,BELGIUM
来源：
JOURNAL OF LIQUID CHROMATOGRAPHY | 1994年 / 17卷 / 09期
关键词：
D O I：
10.1080/10826079408013480
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A high-performance liquid chromatography method for the analysis of propylthiouracil in plasma was validated. Methylthiouracil was used as the internal standard. A one step extraction procedure and an isocratic HPLC method with UV detection were used. No plasma components were found to interfere in the assay. Linear calibration (r(2) > 0.99) curves using water and plasma as matrices in the range of 0.05 to 15 mu g.mL(-1) were obtained. Good recoveries for propylthiouracil (> 85%) and methylthiouracil (> 65%) were seen both in water and in plasma. The coefficient of variation for repeatability was < 7%, for reproducibility < 8% and for accuracy < 6%. The limit of detection was 2 ng.mL(-1) in water and 5 ng.mL(-1) in plasma. A poor stability of propylthiouracil was observed even at -20 degrees C and it is recommended to perform the analyze shortly after sampling.
引用
收藏
页码：2069 / 2083
页数：15
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