CHARACTERIZATION OF CULTURED RAT AORTIC ENDOTHELIAL-CELLS

被引：6
|
作者：
ANDRE, P
MICHEL, M
SCHOTT, C
STOCLET, JC
机构：
[1] Laboratoire de Pharmacologie Cellulaire et Moléculaire, CNRS URA 600, Université Louis Pasteur de Strasbourg, 67401 Illkirch
来源：
JOURNAL OF PHYSIOLOGY-PARIS | 1992年 / 86卷 / 04期
关键词：
ALPHA-SMOOTH-MUSCLE ACTIN; ANGIOTENSIN-II; ATP; BRADYKININ; ENDOTHELIUM DERIVED RELAXING FACTOR; INTRACELLULAR CALCIUM; RAT AORTA; ENDOTHELIAL CELLS;
D O I：
10.1016/0928-4257(92)90004-Y
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
We describe a new method to obtain rat aortic endothelial cells without contamination by vascular smooth muscle cells. The endothelial cells were characterized up to the 20th passage by low density lipoprotein incorporation, the absence of alpha-smooth muscle actin, the production of endothelium derived relaxing factor, and an elevation in intracellular free calcium concentration in response to bradykinin and ATP but not to AMP and angiotensin II.
引用
收藏
页码：177 / 184
页数：8
相关论文
共 50 条
  • [31] RAT AORTIC ENDOTHELIAL-CELLS MIGRATE TOWARD THE HEART
    KIOSSES, WB
    MCKEE, NH
    KALNINS, VI
    MOLECULAR BIOLOGY OF THE CELL, 1995, 6 : 1519 - 1519
  • [32] CHARACTERIZATION OF KININ RECEPTORS ON BOVINE AORTIC ENDOTHELIAL-CELLS
    DORLEANSJUSTE, P
    DENUCCI, G
    REGOLI, D
    VANE, JR
    BRITISH JOURNAL OF PHARMACOLOGY, 1988, 95 : P546 - P546
  • [33] ISOLATION AND CHARACTERIZATION OF RAT LYMPHATIC ENDOTHELIAL-CELLS
    DJONEIDI, M
    BRODT, P
    MICROCIRCULATION ENDOTHELIUM AND LYMPHATICS, 1991, 7 (4-6) : 161 - 182
  • [34] A LARGE CONDUCTANCE ANION CHANNEL IN CULTURED BOVINE AORTIC ENDOTHELIAL-CELLS
    MACGREGOR, GG
    KEMP, PJ
    JOURNAL OF PHYSIOLOGY-LONDON, 1992, 452 : P305 - P305
  • [35] REGULATION OF PROSTAGLANDIN PRODUCTION AND ECTOENZYME ACTIVITIES IN CULTURED AORTIC ENDOTHELIAL-CELLS
    CHESTERMAN, CN
    AGER, A
    GORDON, JL
    JOURNAL OF CELLULAR PHYSIOLOGY, 1983, 116 (01) : 45 - 50
  • [36] METABOLIC ASPECTS OF ENDOTHELIAL-CELLS CULTURED FROM RAT AORTAE
    HASHIMOTO, M
    HARA, T
    HONDA, M
    ISHINAGA, Y
    MORIYAMA, K
    MASUMURA, S
    ARTERY, 1992, 19 (05) : 284 - 296
  • [37] ADHESION OF ERYSIPELOTHRIX RHUSIOPATHIAE TO CULTURED RAT AORTIC ENDOTHELIAL-CELLS - ROLE OF BACTERIAL NEURAMINIDASE IN THE INDUCTION OF ARTERITIS
    NAKATO, H
    SHINOMIYA, K
    MIKAWA, H
    PATHOLOGY RESEARCH AND PRACTICE, 1987, 182 (02) : 255 - 260
  • [38] EICOSANOID MODULATION OF STRESS FIBERS IN CULTURED BOVINE AORTIC ENDOTHELIAL-CELLS
    WELLES, SL
    SHEPRO, D
    HECHTMAN, HB
    INFLAMMATION, 1985, 9 (04) : 439 - 450
  • [39] IMMUNOHISTOLOGIC LOCALIZATION IN AORTA AND BIOSYNTHESIS BY CULTURED HUMAN AORTIC ENDOTHELIAL-CELLS
    TOKUNAGA, O
    FAN, JL
    WATANABE, T
    KOBAYASHI, M
    KUMAZAKI, T
    MITSUI, Y
    LABORATORY INVESTIGATION, 1992, 67 (02) : 210 - 217
  • [40] INFLAMMATORY EICOSANOIDS DECREASE STRESS FIBERS IN CULTURED AORTIC ENDOTHELIAL-CELLS
    WELLES, SL
    SHEPRO, D
    HECHTMAN, HB
    CIRCULATORY SHOCK, 1985, 16 (01) : 52 - 52
12345