CHARACTERIZATION OF CULTURED RAT AORTIC ENDOTHELIAL-CELLS

被引：6
|
作者：
ANDRE, P
MICHEL, M
SCHOTT, C
STOCLET, JC
机构：
[1] Laboratoire de Pharmacologie Cellulaire et Moléculaire, CNRS URA 600, Université Louis Pasteur de Strasbourg, 67401 Illkirch
来源：
JOURNAL OF PHYSIOLOGY-PARIS | 1992年 / 86卷 / 04期
关键词：
ALPHA-SMOOTH-MUSCLE ACTIN; ANGIOTENSIN-II; ATP; BRADYKININ; ENDOTHELIUM DERIVED RELAXING FACTOR; INTRACELLULAR CALCIUM; RAT AORTA; ENDOTHELIAL CELLS;
D O I：
10.1016/0928-4257(92)90004-Y
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
We describe a new method to obtain rat aortic endothelial cells without contamination by vascular smooth muscle cells. The endothelial cells were characterized up to the 20th passage by low density lipoprotein incorporation, the absence of alpha-smooth muscle actin, the production of endothelium derived relaxing factor, and an elevation in intracellular free calcium concentration in response to bradykinin and ATP but not to AMP and angiotensin II.
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页码：177 / 184
页数：8
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