METALLOPROTEINASE FROM THE SEEDLINGS OF KALE (BRASSICA-OLERACEA L VAR SABELLICA) - PREPARATION, PARTIAL CHARACTERIZATION AND SUBSTRATE-SPECIFICITY

被引：5
|
作者：
WILIMOWSKAPELC, A [1 ]
DRYJANSKI, M [1 ]
ZAL, T [1 ]
WILUSZ, T [1 ]
机构：
[1] POLISH ACAD SCI,INST IMMUNOL & EXPTL THERAPY,PL-53114 WROCLAW,POLAND
来源：
PLANTA | 1991年 / 185卷 / 03期
关键词：
BRASSICA; METALLOPROTEINASE; PROTEOLYTIC SPECIFICITY (ENZYME);
D O I：
10.1007/BF00201054
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Metallo-proteinase from 8-d-old seedlings of kale was isolated. The enzyme was extracted with 1% NaCl, concentrated by ammonium sulfate and finally purified by high-performance liquid chromatography. The isolated enzyme had a molecular weight of 22.4 kDa and showed a maximum activity of pH 9.0 using casein as a substrate. Proteolytic activity of proteinase was inhibited by chelators. The inhibition by ethylenediaminetetraacetate (EDTA) was abolished by some divalent metals ions, especially by Zn2+. The enzyme showed activity against the synthetic peptides Suc-Ala-Ala-Pro-Leu-pNA and Suc-Ala-Ala-Pro-Phe-pNA, and hydrolized the following peptide bonds in the oxidized insulin B-chain: Leu6-Cya7, Leu15-Tyr16, Leu17-Val18 and Phe25-Tyr26.
引用
收藏
页码：344 / 349
页数：6
相关论文
共 50 条
  • [31] Influence of Cultivar and Fertilizer Approach on Curly Kale (Brassica oleracea L. var. sabellica). 1. Genetic Diversity Reflected in Agronomic Characteristics and Phytochemical Concentration
    Groenbaek, Marie
    Jensen, Sidsel
    Neugar, Susanne
    Schreiner, Monika
    Kidmose, Ulla
    Kristensen, Hanne Lakkenborg
    JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2014, 62 (47) : 11393 - 11402
  • [32] Potential Biological Activities of Peptides Generated during Casein Proteolysis by Curly Kale (Brassica oleracea L. var. sabellica L.) Leaf Extract: An In Silico Preliminary Study
    Polak-Berecka, Magdalena
    Michalak-Tomczyk, Magdalena
    Skrzypczak, Katarzyna
    Michalak, Katarzyna
    Rachwal, Kamila
    Wasko, Adam
    FOODS, 2021, 10 (11)
  • [33] OBSERVATIONS ON ENTOMOPHILOUS POLLINATION OF A FEW PAIRS OF SINGLE HYBRIDS OF FODDER KALE (BRASSICA-OLERACEA L VAR ACEPHALA DC) IN ISOLATION FOR THE PRODUCTION OF DOUBLE HYBRID SEEDS
    MESQUIDA, J
    APIDOLOGIE, 1978, 9 (04) : 321 - 339
  • [34] S-METHYL METHANE THIOSULFONATE, A NEW ANTIMUTAGENIC COMPOUND ISOLATED FROM BRASSICA-OLERACEA L VAR BOTRYTIS
    NAKAMURA, Y
    MATSUO, T
    SHIMOI, K
    NAKAMURA, Y
    TOMITA, I
    BIOLOGICAL & PHARMACEUTICAL BULLETIN, 1993, 16 (02) : 207 - 209
  • [35] CLONING AND SEQUENCE-ANALYSIS OF A FLO/LFY HOMOLOG ISOLATED FROM CAULIFLOWER (BRASSICA-OLERACEA L VAR BOTRYTIS)
    ANTHONY, RG
    JAMES, PE
    JORDAN, BR
    PLANT MOLECULAR BIOLOGY, 1993, 22 (06) : 1163 - 1166
  • [36] PURIFICATION, PARTIAL CHARACTERIZATION AND SUBSTRATE-SPECIFICITY OF A SQUALENE CYCLASE FROM BACILLUS-ACIDOCALDARIUS
    NEUMANN, S
    SIMON, H
    BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1986, 367 (08): : 723 - 729
  • [37] PARTIAL-PURIFICATION AND CHARACTERIZATION OF CYSTINE LYASE FROM CABBAGE (BRASSICA-OLERACEA-VAR-CAPITATA)
    HALL, DI
    SMITH, IK
    PLANT PHYSIOLOGY, 1983, 72 (03) : 654 - 658
  • [38] Physicochemical Contents, Antioxidant Activities, and Acute Toxicity Assessment of Selenium-Enriched Chinese Kale (Brassica oleracea var. alboglabra L.) Seedlings
    Leamsamrong, Kwanyuen
    Tongjaroenbuangam, Walaiporn
    Maneetong, Sarunya
    Chantiratikul, Anut
    Chinrasri, Orawan
    Chantiratikul, Piyanete
    JOURNAL OF CHEMISTRY, 2019, 2019
  • [39] ISOLATION AND PURIFICATION OF SUPEROXIDE-DISMUTASE PURIFIED FROM BRUSSELS-SPROUTS (BRASSICA-OLERACEA L VAR BULLATA-SUB VAR GEMMIFERA)
    WALKER, JL
    MCLELLAN, KM
    ROBINSON, DS
    FOOD CHEMISTRY, 1991, 41 (01) : 1 - 9
  • [40] EFFECT OF RADIATION ON BIOSYNTHESIS OF L-TRYPTOPHAN FROM C-14-ANTHRANILIC ACID IN KOHLRABI (BRASSICA-OLERACEA L, VAR GONGYLODES L)
    EDER, J
    KUTACEK, M
    BIOLOGIA PLANTARUM, 1972, 14 (06) : 390 - &
12345