METALLOPROTEINASE FROM THE SEEDLINGS OF KALE (BRASSICA-OLERACEA L VAR SABELLICA) - PREPARATION, PARTIAL CHARACTERIZATION AND SUBSTRATE-SPECIFICITY

被引：5

作者：

WILIMOWSKAPELC, A ^{[1
]}

DRYJANSKI, M ^{[1
]}

ZAL, T ^{[1
]}

WILUSZ, T ^{[1
]}

机构：

[1] POLISH ACAD SCI,INST IMMUNOL & EXPTL THERAPY,PL-53114 WROCLAW,POLAND

来源：

PLANTA | 1991年 / 185卷 / 03期

关键词：

BRASSICA; METALLOPROTEINASE; PROTEOLYTIC SPECIFICITY (ENZYME);

D O I：

10.1007/BF00201054

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Metallo-proteinase from 8-d-old seedlings of kale was isolated. The enzyme was extracted with 1% NaCl, concentrated by ammonium sulfate and finally purified by high-performance liquid chromatography. The isolated enzyme had a molecular weight of 22.4 kDa and showed a maximum activity of pH 9.0 using casein as a substrate. Proteolytic activity of proteinase was inhibited by chelators. The inhibition by ethylenediaminetetraacetate (EDTA) was abolished by some divalent metals ions, especially by Zn2+. The enzyme showed activity against the synthetic peptides Suc-Ala-Ala-Pro-Leu-pNA and Suc-Ala-Ala-Pro-Phe-pNA, and hydrolized the following peptide bonds in the oxidized insulin B-chain: Leu6-Cya7, Leu15-Tyr16, Leu17-Val18 and Phe25-Tyr26.

引用

页码：344 / 349

页数：6

共 50 条

[1] ISOLATION AND PARTIAL CHARACTERIZATION OF THE TRYPSIN-INHIBITOR FROM THE SEEDS OF BRASSICA-OLERACEA VAR SABELLICA
WILIMOWSKAPELC, A
ACTA BIOCHIMICA POLONICA, 1985, 32 (04) : 351 - &
[2] Yield and Quality Improvement of Curly Kale (Brassica oleracea var. Sabellica L.) by Utilizing Agricultural Waste
Sembiring, Gitta Malinda
Karyawati, Anna Satyana
Maghfoer, Mochammad Dawam
JOURNAL OF ECOLOGICAL ENGINEERING, 2023, 24 (04): : 163 - 171
[3] SELECTION FOR RESISTANCE TO CLUBROOT (PLASMODIOPHORA-BRASSICAE) IN MARROWSTEM KALE (BRASSICA-OLERACEA VAR ACEPHALA L)
BRADSHAW, JE
WILLIAMSON, CJ
ANNALS OF APPLIED BIOLOGY, 1991, 119 (03) : 501 - 511
[4] Kale (Brassica oleracea L. var. sabellica) biofortified with iodoquinolines: Effectiveness of enriching with iodine and influence on chemical composition
Krawczyk, Katarzyna Karolina
Smolen, Sylwester
Wisla-Swider, Anna
Kowalska, Iwona
Kielbasa, Daniel
Pitala, Joanna
Krzeminska, Joanna
Wasniowska, Justyna
Koronowicz, Aneta
SCIENTIA HORTICULTURAE, 2024, 323
[5] ISOLATION AND CHARACTERIZATION OF A PARTIAL CDNA CLONE FOR UBIQUITIN-CONJUGATING ENZYME FROM BROCCOLI (BRASSICA-OLERACEA L VAR ITALICA)
SUH, MC
SONG, HW
CHO, HS
YI, HK
HONG, CB
MOLECULES AND CELLS, 1995, 5 (03) : 293 - 297
[6] DISSIPATION OF PARATHION RESIDUES FROM CABBAGE (BRASSICA-OLERACEA L VAR CAPITATA L)
SINGH, J
KRUEGER, HR
DESHMUKH, SN
BINDRA, OS
INDIAN JOURNAL OF AGRICULTURAL SCIENCES, 1971, 41 (04): : 328 - &
[7] HAPLOID PRODUCTION FROM ANTHER CULTURES OF BRASSICA-OLERACEA L VAR CAPITATA AND VAR GEMMIFERA
LELU, MA
BOLLON, H
COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE III-SCIENCES DE LA VIE-LIFE SCIENCES, 1985, 300 (02): : 71 - &
[8] Dough Rheological Properties and Characteristics of Wheat Bread with the Addition of Lyophilized Kale (Brassica oleracea L. var. sabellica) Powder
Korus, Anna
Witczak, Mariusz
Korus, Jaroslaw
Juszczak, Leslaw
APPLIED SCIENCES-BASEL, 2023, 13 (01):
[9] Recovery of Alternaria brassicicola from Chopped, Bagged Kale (Brassica oleracea var. sabellica) (vol 108, pg 2989, 2024)
Toporek, S. M.
PLANT DISEASE, 2024, 108 (10)
[10] Highly glycosylated and acylated flavonols isolated from kale (Brassica oleracea var. sabellica) - Structure-antioxidant activity relationship
Fiol, Michaela
Adermann, Susann
Neugart, Susanne
Rohn, Sascha
Muegge, Clemens
Schreiner, Monika
Krumbein, Angelika
Kroh, Lothar W.
FOOD RESEARCH INTERNATIONAL, 2012, 47 (01) : 80 - 89

← 1 2 3 4 5 →