RAPID METHOD FOR THE ENRICHMENT OF VERY LONG-CHAIN FATTY-ACIDS FROM MICROORGANISMS

被引:0
|
作者
REZANKA, T [1 ]
SOKOLOV, MY [1 ]
机构
[1] MOSCOW MICROBIOL INST, MOSCOW 117811, RUSSIA
来源
JOURNAL OF CHROMATOGRAPHY | 1993年 / 636卷 / 02期
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D O I
暂无
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A method for the enrichment very long-chain fatty acids (VLCFAs) from total fatty acid-containing samples is described. The method is based on the use of reversed-phase high-performance liquid chromatography and/or solid-phase extraction cartridges with subsequent identification of VLCFAs by capillary gas chromatography-mass spectrometry. By combining the two techniques, unusual VLCFAs (higher than C22) were identified in newly isolated oligotrophic bacteria. VLCFAs have already been described in sulphate-reducing bacteria and in some wood-decaying fungi.
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页码:249 / 254
页数:6
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