RESOLUTION OF THE CONFORMATIONAL DISTRIBUTION AND DYNAMICS OF A FLEXIBLE MOLECULE USING FREQUENCY-DOMAIN FLUOROMETRY

被引：25

作者：

LAKOWICZ, JR ^{[1
]}

KUSBA, J ^{[1
]}

WICZK, W ^{[1
]}

GRYCZYNSKI, I ^{[1
]}

SZMACINSKI, H ^{[1
]}

JOHNSON, ML ^{[1
]}

机构：

[1] UNIV VIRGINIA,DEPT PHARMACOL,CHARLOTTESVILLE,VA 22908

来源：

BIOPHYSICAL CHEMISTRY | 1991年 / 39卷 / 01期

关键词：

FLUORESCENCE; MOLECULAR DYNAMICS; CONFORMATIONAL ANALYSIS; RESONANCE ENERGY TRANSFER; FREQUENCY-DOMAIN FLUOROMETRY;

D O I：

10.1016/0301-4622(91)85008-E

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the first resolution of both the conformational distribution and end-to-end diffusion coefficient of a flexible molecule. This molecular information was recovered using only the donor intensity decay in a single solvent at a single viscosity, as observed by the technique of frequency-domain fluorometry. This technique can be extended to meaurements of structural fluctuations of biological macromolecules.

引用

页码：79 / 84

页数：6

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