RESOLUTION OF THE CONFORMATIONAL DISTRIBUTION AND DYNAMICS OF A FLEXIBLE MOLECULE USING FREQUENCY-DOMAIN FLUOROMETRY

被引：25
|
作者：
LAKOWICZ, JR [1 ]
KUSBA, J [1 ]
WICZK, W [1 ]
GRYCZYNSKI, I [1 ]
SZMACINSKI, H [1 ]
JOHNSON, ML [1 ]
机构：
[1] UNIV VIRGINIA,DEPT PHARMACOL,CHARLOTTESVILLE,VA 22908
来源：
BIOPHYSICAL CHEMISTRY | 1991年 / 39卷 / 01期
关键词：
FLUORESCENCE; MOLECULAR DYNAMICS; CONFORMATIONAL ANALYSIS; RESONANCE ENERGY TRANSFER; FREQUENCY-DOMAIN FLUOROMETRY;
D O I：
10.1016/0301-4622(91)85008-E
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We report the first resolution of both the conformational distribution and end-to-end diffusion coefficient of a flexible molecule. This molecular information was recovered using only the donor intensity decay in a single solvent at a single viscosity, as observed by the technique of frequency-domain fluorometry. This technique can be extended to meaurements of structural fluctuations of biological macromolecules.
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页码：79 / 84
页数：6
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