CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF PSOPHOCARPIN-B1, A CHYMOTRYPSIN INHIBITOR FROM WINGED BEAN-SEEDS

被引：11
|
作者：
DATTAGUPTA, JK [1 ]
CHAKRABARTI, C [1 ]
PODDER, A [1 ]
机构：
[1] INDIAN INST CHEM BIOL,DIV BIOCHEM ENGN,CALCUTTA 700032,W BENGAL,INDIA
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 216卷 / 02期
关键词：
D O I：
10.1016/S0022-2836(05)80312-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Psophocarpin B1 is a 20,000 Mr protein of winged bean (Psophocarpus tetragonolobus) seeds having chymotrypsin inhibitory activity. Single crystals of this protein suitable for X-ray crystallographic studies have been obtained by the vapour diffusion method using ammonium sulphate. The crystals are hexagonal, space group P6422 or P6222, cell dimensions a=b=61 Å, c=210 Å. They are stable to irradiation with X-rays and diffract to at least 2·6 Å resolution. © 1990 Academic Press Limited.
引用
收藏
页码：229 / 231
页数：3
相关论文
共 50 条
  • [31] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF THE LECTIN FROM ERYTHRINA-CORALLODENDRON
    SAPER, MA
    LIS, H
    SHARON, N
    SHAANAN, B
    JOURNAL OF MOLECULAR BIOLOGY, 1987, 193 (04) : 823 - 824
  • [32] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF A BACTERIAL FLAVOHEMOGLOBIN PROTEIN
    ERMLER, U
    SIDDIQUI, RA
    CRAMM, R
    SCHRODER, D
    FRIEDRICH, B
    PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1995, 21 (04): : 351 - 353
  • [33] CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF HUMAN ERYTHROCYTE ACYLPEPTIDE HYDROLASE
    FEESE, M
    SCALONI, A
    JONES, WM
    MANNING, JM
    REMINGTON, SJ
    JOURNAL OF MOLECULAR BIOLOGY, 1993, 233 (03) : 546 - 549
  • [34] CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF HUMAN VASCULAR ANTICOAGULANT PROTEIN
    LEWITBENTLEY, A
    DOUBLIE, S
    FOURME, R
    BODO, G
    JOURNAL OF MOLECULAR BIOLOGY, 1989, 210 (04) : 875 - 876
  • [35] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF A PEROXIDASE FROM BARLEY-GRAIN
    HENRIKSEN, A
    PETERSEN, JFW
    SVENSSON, A
    HEJGAARD, J
    WELINDER, KG
    GAJHEDE, M
    JOURNAL OF MOLECULAR BIOLOGY, 1992, 228 (02) : 690 - 692
  • [36] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF THE ALPHA-AMYLASE INHIBITOR CODED 0.19 FROM WHEAT KERNEL
    MIYAZAKI, T
    MORIMOTO, T
    FUKUYAMA, K
    MATSUBARA, H
    JOURNAL OF BIOCHEMISTRY, 1994, 115 (02): : 179 - 181
  • [37] CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF TOBACCO NECROSIS VIRUS
    FUKUYAMA, K
    HIROTA, S
    TSUKIHARA, T
    JOURNAL OF MOLECULAR BIOLOGY, 1987, 196 (04) : 961 - 962
  • [38] CRYSTALLIZATION AND PRELIMINARY-X-RAY CRYSTALLOGRAPHIC ANALYSIS OF VEROTOXIN-1 B-SUBUNIT
    BOODHOO, A
    READ, RJ
    BRUNTON, J
    JOURNAL OF MOLECULAR BIOLOGY, 1991, 221 (03) : 729 - 731
  • [39] Structure of a Kunitz-type chymotrypsin inhibitor from winged bean seeds at 2.95 angstrom resolution
    Dattagupta, JK
    Podder, A
    Chakrabarti, C
    Sen, U
    Dutta, SK
    Singh, M
    ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY, 1996, 52 : 521 - 528
  • [40] CRYSTALLIZATION AND PRELIMINARY-X-RAY CRYSTALLOGRAPHIC STUDIES OF NONHISTONE REGION OF MACROH2A.1
    VIJAYKUMAR, S
    CHANDRA, N
    DHARIA, C
    PEHRSON, JR
    PROTEINS-STRUCTURE FUNCTION AND GENETICS, 1995, 22 (03): : 290 - 292
12345