CRYSTALLIZATION AND PRELIMINARY-X-RAY STUDIES OF PSOPHOCARPIN-B1, A CHYMOTRYPSIN INHIBITOR FROM WINGED BEAN-SEEDS

被引：11
|
作者：
DATTAGUPTA, JK [1 ]
CHAKRABARTI, C [1 ]
PODDER, A [1 ]
机构：
[1] INDIAN INST CHEM BIOL,DIV BIOCHEM ENGN,CALCUTTA 700032,W BENGAL,INDIA
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 216卷 / 02期
关键词：
D O I：
10.1016/S0022-2836(05)80312-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Psophocarpin B1 is a 20,000 Mr protein of winged bean (Psophocarpus tetragonolobus) seeds having chymotrypsin inhibitory activity. Single crystals of this protein suitable for X-ray crystallographic studies have been obtained by the vapour diffusion method using ammonium sulphate. The crystals are hexagonal, space group P6422 or P6222, cell dimensions a=b=61 Å, c=210 Å. They are stable to irradiation with X-rays and diffract to at least 2·6 Å resolution. © 1990 Academic Press Limited.
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收藏
页码：229 / 231
页数：3
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