CHEMILUMINOMETRIC L-LYSINE DETERMINATION WITH IMMOBILIZED LYSINE OXIDASE BY FLOW-INJECTION ANALYSIS

被引：34
|
作者：
PREUSCHOFF, F
SPOHN, U
WEBER, E
UNVERHAU, K
MOHR, KH
机构：
[1] UNIV HALLE,INST BIOTECHNOL,WEINBERGWEG 16A,O-4050 HALLE,GERMANY
[2] UNIV HALLE,DEPT MED,INST BIOCHEM,O-4020 HALLE,GERMANY
[3] UNIV HALLE,INST MICROBIOL,O-4050 HALLE,GERMANY
来源：
ANALYTICA CHIMICA ACTA | 1993年 / 280卷 / 02期
关键词：
CHEMILUMINESCENCE; ENZYMATIC METHODS; FLOW INJECTION; LYSINE;
D O I：
10.1016/0003-2670(93)85120-9
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An enzymatic flow-injection procedure for the determination of L-lysine was developed. A lysine oxidase reactor is combined with a fibre-optic hydrogen peroxide detector. Hydrogen peroxide detection is based on the peroxidase-catalysed luminol reaction. The chemiluminescent light is detected by a photomultiplier. L-Lysine can be determined in the range 10-1000 muM. A sampling rate of up to 90 h-1 can be achieved. The whole sensing assay works for more than 1 month. The double logarithmic graph of the peak signal height vs. the lysine concentration is linear, the slope being larger than unity (r2 = 0.991, n = 4).
引用
收藏
页码：185 / 189
页数：5
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