TRANSFORMATION OF ASPERGILLUS-ORYZAE WITH A DOMINANT SELECTABLE MARKER

被引：8
|
作者：
CHEEVADHANARAK, S [1 ]
SAUNDERS, G [1 ]
RENNO, DV [1 ]
FLEGEL, TW [1 ]
HOLT, G [1 ]
机构：
[1] POLYTECH CENT LONDON, SCH BIOL & HLTH SCI, 115 NEW CAVENDISH ST, LONDON W1M 8JS, ENGLAND
来源：
JOURNAL OF BIOTECHNOLOGY | 1991年 / 19卷 / 01期
关键词：
ASPERGILLUS-ORYZAE; PHLEOMYCIN RESISTANCE; TRANSFORMATION;
D O I：
10.1016/0168-1656(91)90079-B
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aspergillus oryzae strain U1638 has been transformed to phleomycin resistance using a phleomycin based vector. Protoplasts were generated using Novozyme 234 and regenerated on sucrose-stabilized minimal medium containing 200-mu-g ml-1 phleomycin. A frequency of 3 to 7 transformants per 10(6) viable protoplasts was obtained. All transformants tested showed increased resistance to phleomycin when subcultured onto selective medium and the mitotic stability of the transformants was 100% after growth without selective pressure. The vector sequences appeared to be integrated into the A. oryzae U1638 genome at multiple sites.
引用
收藏
页码：117 / 122
页数：6
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