TRANSFORMATION OF ASPERGILLUS-ORYZAE WITH A DOMINANT SELECTABLE MARKER

被引：8
|
作者：
CHEEVADHANARAK, S [1 ]
SAUNDERS, G [1 ]
RENNO, DV [1 ]
FLEGEL, TW [1 ]
HOLT, G [1 ]
机构：
[1] POLYTECH CENT LONDON, SCH BIOL & HLTH SCI, 115 NEW CAVENDISH ST, LONDON W1M 8JS, ENGLAND
来源：
JOURNAL OF BIOTECHNOLOGY | 1991年 / 19卷 / 01期
关键词：
ASPERGILLUS-ORYZAE; PHLEOMYCIN RESISTANCE; TRANSFORMATION;
D O I：
10.1016/0168-1656(91)90079-B
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aspergillus oryzae strain U1638 has been transformed to phleomycin resistance using a phleomycin based vector. Protoplasts were generated using Novozyme 234 and regenerated on sucrose-stabilized minimal medium containing 200-mu-g ml-1 phleomycin. A frequency of 3 to 7 transformants per 10(6) viable protoplasts was obtained. All transformants tested showed increased resistance to phleomycin when subcultured onto selective medium and the mitotic stability of the transformants was 100% after growth without selective pressure. The vector sequences appeared to be integrated into the A. oryzae U1638 genome at multiple sites.
引用
收藏
页码：117 / 122
页数：6
相关论文
共 50 条
  • [31] PROPERTIES OF CHYMOTRYPSIN PROTEINASE FROM ASPERGILLUS-ORYZAE
    KOLODZEJSKA, MV
    TSYPEROVYCH, OS
    SKURATOVSKA, ND
    UKRAINSKII BIOKHIMICHESKII ZHURNAL, 1977, 49 (03): : 42 - 46
  • [32] CELLULAR LOCALIZATION OF ACID CARBOXYPEPTIDASE IN ASPERGILLUS-ORYZAE
    NOMI, T
    MIKAMI, S
    YAMANE, A
    TAKEUCHI, M
    ICHISHIMA, E
    CURRENT MICROBIOLOGY, 1978, 1 (01) : 25 - 28
  • [33] INHIBITION STUDY OF TYROSINASE FROM ASPERGILLUS-ORYZAE
    TANAKA, T
    TAKEUCHI, M
    ICHISHIMA, E
    AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1989, 53 (02): : 557 - 558
  • [34] PHLEOMYCIN RESISTANCE AS A DOMINANT SELECTABLE MARKER FOR PLANT-CELL TRANSFORMATION
    PEREZ, P
    TIRABY, G
    KALLERHOFF, J
    PERRET, J
    PLANT MOLECULAR BIOLOGY, 1989, 13 (04) : 365 - 373
  • [35] HYDROLYTIC DEGRADATION OF CAPSAICIN BY ASPERGILLUS-NIGER AND ASPERGILLUS-ORYZAE
    ONOZAKI, H
    SASAOKA, K
    EZAKI, H
    JOURNAL OF FERMENTATION TECHNOLOGY, 1976, 54 (05): : 297 - 301
  • [36] CRYSTALLIZATION OF THE ALPHA-AMYLASE OF ASPERGILLUS-ORYZAE
    FISCHER, EH
    DEMONTMOLLIN, R
    NATURE, 1951, 168 (4275) : 606 - 607
  • [37] ASPERGILLUS-ORYZAE (NRRL STRAIN 1988) - CLARIFICATION
    FENNELL, DI
    SCIENCE, 1976, 194 (4270) : 1188 - 1188
  • [38] PURIFICATION AND PROPERTIES OF GLUTAMINASE FROM ASPERGILLUS-ORYZAE
    YANO, T
    ITO, M
    TOMITA, K
    KUMAGAI, H
    TOCHIKURA, T
    JOURNAL OF FERMENTATION TECHNOLOGY, 1988, 66 (02): : 137 - 143
  • [39] OCCUPATIONAL EXTRINSIC ALLERGIC ALVEOLITIS TO ASPERGILLUS-ORYZAE
    GUELAUD, C
    FRUIT, J
    VANNIMENUS, C
    WALLAERT, B
    TONNEL, AB
    REVUE DES MALADIES RESPIRATOIRES, 1993, 10 (04) : 362 - 365
  • [40] ISOLATION AND PROPERTIES OF THE SERINE PROTEINASE OF ASPERGILLUS-ORYZAE
    VAGANOVA, TI
    IVANOVA, NM
    KHODOVA, OM
    VOYUSHINA, TL
    STEPANOV, VM
    BIOCHEMISTRY-MOSCOW, 1991, 56 (01) : 94 - 101
12345