A CYTOFLUOROMETRIC METHOD TO QUANTIFY MEMBRANE-ANTIGENS ON INDIVIDUAL ALVEOLAR MACROPHAGES

被引：4
|
作者：
PANKOW, W [1 ]
NEUMANN, K [1 ]
RUSCHOFF, J [1 ]
HEYMANNS, J [1 ]
VONWICHERT, P [1 ]
机构：
[1] UNIV HOSP MARBURG,DEPT PATHOL,W-3550 MARBURG,GERMANY
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 129卷 / 01期
关键词：
Alveolar macrophage; Cytophotometry; HLA-DR antigen; Immunofluorescence; Monoclonal antibody;
D O I：
10.1016/0022-1759(90)90429-Y
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An immunofluorescence method for assaying membrane-bound antigens on individual alveolar macrophages (AM) collected by bronchoalveolar lavage (BAL) is described. Cells were labelled with FITC-conjugated anti-HLA-DR antibodies in a single (direct) step. Quantification of the fluorescence was performedv by computer-assisted cytophotometry. Alveolar macrophages, especially when obtained from cigarette smokers, exhibited an autofluorescence which interfered with the measurement of specific fluorescence. The specific fluorescence was calculated by determination of total fluorescence in the wave-length optimal for FITC and the non-specific fluorescence in a different wavelength during a second measurement. Specificity and reproducibility testing confirmed the reliability of the method. © 1990.
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页码：127 / 133
页数：7
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