A CYTOFLUOROMETRIC METHOD TO QUANTIFY MEMBRANE-ANTIGENS ON INDIVIDUAL ALVEOLAR MACROPHAGES

被引：4
|
作者：
PANKOW, W [1 ]
NEUMANN, K [1 ]
RUSCHOFF, J [1 ]
HEYMANNS, J [1 ]
VONWICHERT, P [1 ]
机构：
[1] UNIV HOSP MARBURG,DEPT PATHOL,W-3550 MARBURG,GERMANY
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 129卷 / 01期
关键词：
Alveolar macrophage; Cytophotometry; HLA-DR antigen; Immunofluorescence; Monoclonal antibody;
D O I：
10.1016/0022-1759(90)90429-Y
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
An immunofluorescence method for assaying membrane-bound antigens on individual alveolar macrophages (AM) collected by bronchoalveolar lavage (BAL) is described. Cells were labelled with FITC-conjugated anti-HLA-DR antibodies in a single (direct) step. Quantification of the fluorescence was performedv by computer-assisted cytophotometry. Alveolar macrophages, especially when obtained from cigarette smokers, exhibited an autofluorescence which interfered with the measurement of specific fluorescence. The specific fluorescence was calculated by determination of total fluorescence in the wave-length optimal for FITC and the non-specific fluorescence in a different wavelength during a second measurement. Specificity and reproducibility testing confirmed the reliability of the method. © 1990.
引用
收藏
页码：127 / 133
页数：7
相关论文
共 50 条
  • [21] MEMBRANE-ANTIGENS AND CELL POSITIONS IN THE APPENDIX OF AN INSECT
    BULLIERE, D
    BULLIERE, F
    MOUNAJI, K
    DEREGGI, M
    GHARIB, B
    ARCHIVES D ANATOMIE MICROSCOPIQUE ET DE MORPHOLOGIE EXPERIMENTALE, 1982, 71 (01): : 75 - 76
  • [22] LYSOSOMAL MEMBRANE-ANTIGENS IN THE NEUTROPHIL PLASMA-MEMBRANE FOLLOWING SECRETION
    KLEMPNER, MS
    MIKKELSEN, RB
    CLINICAL RESEARCH, 1983, 31 (02): : A368 - A368
  • [23] DETERMINATION OF MEMBRANE-ANTIGENS BY A COVALENT CROSS-LINKING METHOD WITH MONOCLONAL-ANTIBODIES
    HAMADA, H
    TSURUO, T
    ANALYTICAL BIOCHEMISTRY, 1987, 160 (02) : 483 - 488
  • [24] TRANSFECTION AND CLONING OF GENES FOR MEMBRANE-ANTIGENS USING THE FACS
    HERZENBERG, LA
    HSU, C
    ALBERTI, S
    KAVATHAS, P
    MEDICAL ONCOLOGY AND TUMOR PHARMACOTHERAPY, 1984, 1 (04): : 219 - 224
  • [25] DETECTION AND CHARACTERIZATION OF MEMBRANE-ANTIGENS OF TOXOPLASMA-GONDII
    HANDMAN, E
    GODING, JW
    REMINGTON, JS
    JOURNAL OF IMMUNOLOGY, 1980, 124 (06): : 2578 - 2583
  • [26] USE OF MEMBRANE-ANTIGENS AND EXO-ANTIGENS FOR IMMUNODIAGNOSIS OF HUMAN SCHISTOSOMIASIS
    ROTMANS, JP
    MOOIJ, GW
    TROPICAL AND GEOGRAPHICAL MEDICINE, 1979, 31 (01): : 170 - 171
  • [27] ANTIBODIES TO MEMBRANE-ANTIGENS IN AUTOIMMUNE THYROID-DISEASE
    BRADBURY, J
    FURMANIAK, J
    ANNALES D ENDOCRINOLOGIE, 1987, 48 (02) : 146 - 146
  • [28] ANTIBODIES TO MEMBRANE-ANTIGENS IN AUTOIMMUNE THYROID-DISEASE
    FURMANIAK, J
    BRADBURY, J
    SMITH, BR
    ACTA ENDOCRINOLOGICA, 1987, 116 (01): : 13 - 20
  • [29] AUTOANTIBODY REACTIVE INTEGRAL MEMBRANE-ANTIGENS OF THYMOCYTES AND BRAIN
    NARENDRAN, A
    HOFFMAN, SA
    JOURNAL OF NEUROIMMUNOLOGY, 1987, 16 (01) : 133 - 133
  • [30] A RAPID METHOD FOR DETECTION OF MEMBRANE-ANTIGENS BY IMMUNOFLUORESCENCE AND ITS APPLICATION TO SCREENING HYBRIDOMA ANTIBODIES
    BOUCHEIX, C
    PERROT, JY
    MIRSHAHI, M
    BERNADOU, A
    ROSENFELD, C
    JOURNAL OF IMMUNOLOGICAL METHODS, 1983, 57 (1-3) : 145 - 150
12345