GENERATION OF RECOMBINANT BACULOVIRUS VIA LIPOSOME-MEDIATED TRANSFECTION

被引：0

作者：

HARTIG, PC ^{[1
]}

CARDON, MC ^{[1
]}

KAWANISHI, CY ^{[1
]}

机构：

[1] US EPA,RES TRIANGLE PK,NC 27711

来源：

BIOTECHNIQUES | 1991年 / 11卷 / 03期

关键词：

D O I：

暂无

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Baculovirus expression vectors have become a popular method of producing recombinant proteins. Production of recombinant virus requires the transfection of both the native viral DNA and a transfer plasmid into insect cells where recombination takes place. While several methods of transfecting insect cells exist, we have found liposome-mediated transfection to be highly efficient. Here we detail the protocols and medium needed for efficient, simple transfection of Spodoptera frugiperda cells.

引用

页码：310 / &

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