GENERATION OF RECOMBINANT BACULOVIRUS VIA LIPOSOME-MEDIATED TRANSFECTION

被引：0
|
作者：
HARTIG, PC [1 ]
CARDON, MC [1 ]
KAWANISHI, CY [1 ]
机构：
[1] US EPA,RES TRIANGLE PK,NC 27711
来源：
BIOTECHNIQUES | 1991年 / 11卷 / 03期
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D O I：
暂无
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Baculovirus expression vectors have become a popular method of producing recombinant proteins. Production of recombinant virus requires the transfection of both the native viral DNA and a transfer plasmid into insect cells where recombination takes place. While several methods of transfecting insect cells exist, we have found liposome-mediated transfection to be highly efficient. Here we detail the protocols and medium needed for efficient, simple transfection of Spodoptera frugiperda cells.
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页码：310 / &
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